Active Recombinant Full Length Human CDK3 Protein, GST-tagged

• Cat.No.: CDK3-4540HF
• Product Overview: Full-length human CDK3 [1-305(end) amino acids of accession number NP_001249.1] was co-expressed as N-terminal GST-fusion protein (62kDa) with CyclinE1 [1-410(end) amino acids of accession number NP_001229.1] using baculovirus expression system. GST-CDK3 was purified by using glutathione sepharose chromatography.

Specification

• Source: Insect (sf21)
• Species: Human
• Tag: GST
• Form: 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
• Bio-activity: The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
• Protein length: 1-305(end)
• Purity: 66%
• Applications: SDS-PAGE
• Storage: Store at -80C. Avoid repeating freeze-thaws.

Gene Information

• Gene Name: cyclin-dependent kinase 3
• Official Symbol: CDK3
• Synonyms: CDK3
• Protein Refseq: NP_001249.1
• UniProt ID: Q00526