Active Recombinant Full Length Human CTNNB1 Protein, C-Flag-tagged

• Cat.No.: CTNNB1-21HFL
• Product Overview: Recombinant Full Length Human CTNNB1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: Pull-down assay
• Molecular Mass: 85.3 kDa
• AA Sequence: MATQADLMELDMAMEPDRKAAVSHW QQQSYLDSGIHSGATTTAPSLSGKG NPEEEDVDTSQVLYEWEQGF SQSFTQEQVADIDGQYAMTRAQRVR AAMFPETLDEGMQIPSTQFDAAHPT NVQRLAEPSQMLKHAVVNLI NYQDDAELATRAIPELTKLLNDEDQ VVVNKAAVMVHQLSKKEASRHAIMR SPQMVSAIVRTMQNTNDVET ARCTAGTLHNLSHHREGLLAIFKSG GIPALVKMLGSPVDSVLFYAITTLH NLLLHQEGAKMAVRLAGGLQ KMVALLNKTNVKFLAITTDCLQILA YGNQESKLIILASGGPQALVNIMRT YTYEKLLWTTSRVLKVLSVC SSNKPAIVEAGGMQALGLHLTDPSQ RLVQNCLWTLRNLSDAATKQEGMEG LLGTLVQLLGSDDINVVTCA AGILSNLTCNNYKNKMMVCQVGGIE ALVRTVLRAGDREDITEPAICALRH LTSRHQEAEMAQNAVRLHYG LPVVVKLLHPPSHWPLIKATVGLIR NLALCPANHAPLREQGAIPRLVQLL VRAHQDTQRRTSMGGTQQQF VEGVRMEEIVEGCTGALHILARDVH NRIVIRGLNTIPLFVQLLYSPIENI QRVAAGVLCELAQDKEAAEA IEAEGATAPLTELLHSRNEGVATYA AAVLFRMSEDKPQDYKKRLSVELTS SLFRTEPMAWNETADLGLDI GAQGEPLGYRQDDPSYRSFHSGGYG QDALGMDPMMEHEMGGHHPGADYPV DGLPDLGHAQDLMDGLPPGD
  SNQLAWFDTDLTRTRPLEQKLISEE DLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors
• Protein Pathways: Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Basal cell carcinoma, Colorectal cancer, Endometrial cancer, Focal adhesion, Leukocyte transendothelial migration, Melanogenesis, Pathogenic Escherichia coli infection, Pathways in cancer, Prostate cancer, Thyroid cancer, Tight junction, Wnt signaling pathway
• Publication:
  A Novel Anti-Cancer Agent, 1-(3,5-Dimethoxyphenyl)-4-[(6-Fluoro-2-Methoxyquinoxalin-3-yl)Aminocarbonyl] Piperazine (RX-5902), Interferes With β-Catenin Function Through Y593 Phospho-p68 RNA Helicase (2015)

Gene Information

• Gene Name: CTNNB1 catenin beta 1 [ Homo sapiens (human) ]
• Official Symbol: CTNNB1
• Synonyms: EVR7; CTNNB; MRD19; NEDSDV; armadillo
• Gene ID: 1499
• mRNA Refseq: NM_001904.4
• Protein Refseq: NP_001895.1
• MIM: 116806
• UniProt ID: P35222

Reviews

03/27/2023

Not only does it provide reliable and consistent results due to its high quality and purity, but it also holds significant value in understanding cellular processes and disease mechanisms.

07/28/2017

the CTNNB1 protein offers numerous advantages in trials and research experiments.

06/24/2016

This commitment to excellence ensures that researchers are provided with the most advanced and reliable tools for their investigations.

Q&As

Is the CTNNB1 protein involved in the progression of pancreatic cancer?

Yes, CTNNB1 is implicated in the development and progression of pancreatic cancer, with its dysregulated activity contributing to tumor growth, invasion, and metastasis.

How is the CTNNB1 protein associated with hepatocellular carcinoma (HCC)?

Mutations in the CTNNB1 gene lead to the stabilization and accumulation of β-catenin, contributing to the development of HCC, making it a potential biomarker for the diagnosis and prognosis of HCC.

How is the CTNNB1 protein linked to the prognosis of gastric cancer patients?

Studies have shown that CTNNB1 expression levels can serve as an independent prognostic factor in gastric cancer, with high CTNNB1 expression correlating with poor patient outcomes.

Are there any targeted therapies specifically aimed at CTNNB1 in cancer treatment?

While there are no direct CTNNB1-targeted therapies available, various drugs targeting the Wnt signaling pathway are being developed and tested, which indirectly impact CTNNB1 activity in cancer cells.

How is the expression of CTNNB1 in breast cancer cells associated with patient survival?

High expression levels of CTNNB1 in breast cancer cells have been linked to poor prognosis and shorter overall survival, indicating its potential as a prognostic marker for breast cancer.