Active Recombinant Full Length Human CTNNB1 Protein, C-Flag-tagged

• Cat.No.: CTNNB1-21HFL
• Product Overview: Recombinant Full Length Human CTNNB1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: Pull-down assay
• Molecular Mass: 85.3 kDa
• AA Sequence: MATQADLMELDMAMEPDRKAAVSHW QQQSYLDSGIHSGATTTAPSLSGKG NPEEEDVDTSQVLYEWEQGF SQSFTQEQVADIDGQYAMTRAQRVR AAMFPETLDEGMQIPSTQFDAAHPT NVQRLAEPSQMLKHAVVNLI NYQDDAELATRAIPELTKLLNDEDQ VVVNKAAVMVHQLSKKEASRHAIMR SPQMVSAIVRTMQNTNDVET ARCTAGTLHNLSHHREGLLAIFKSG GIPALVKMLGSPVDSVLFYAITTLH NLLLHQEGAKMAVRLAGGLQ KMVALLNKTNVKFLAITTDCLQILA YGNQESKLIILASGGPQALVNIMRT YTYEKLLWTTSRVLKVLSVC SSNKPAIVEAGGMQALGLHLTDPSQ RLVQNCLWTLRNLSDAATKQEGMEG LLGTLVQLLGSDDINVVTCA AGILSNLTCNNYKNKMMVCQVGGIE ALVRTVLRAGDREDITEPAICALRH LTSRHQEAEMAQNAVRLHYG LPVVVKLLHPPSHWPLIKATVGLIR NLALCPANHAPLREQGAIPRLVQLL VRAHQDTQRRTSMGGTQQQF VEGVRMEEIVEGCTGALHILARDVH NRIVIRGLNTIPLFVQLLYSPIENI QRVAAGVLCELAQDKEAAEA IEAEGATAPLTELLHSRNEGVATYA AAVLFRMSEDKPQDYKKRLSVELTS SLFRTEPMAWNETADLGLDI GAQGEPLGYRQDDPSYRSFHSGGYG QDALGMDPMMEHEMGGHHPGADYPV DGLPDLGHAQDLMDGLPPGD
  SNQLAWFDTDLTRTRPLEQKLISEE DLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors
• Protein Pathways: Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Basal cell carcinoma, Colorectal cancer, Endometrial cancer, Focal adhesion, Leukocyte transendothelial migration, Melanogenesis, Pathogenic Escherichia coli infection, Pathways in cancer, Prostate cancer, Thyroid cancer, Tight junction, Wnt signaling pathway
• Publication:
  A Novel Anti-Cancer Agent, 1-(3,5-Dimethoxyphenyl)-4-[(6-Fluoro-2-Methoxyquinoxalin-3-yl)Aminocarbonyl] Piperazine (RX-5902), Interferes With β-Catenin Function Through Y593 Phospho-p68 RNA Helicase (2015)

Gene Information

• Gene Name: CTNNB1 catenin beta 1 [ Homo sapiens (human) ]
• Official Symbol: CTNNB1
• Synonyms: EVR7; CTNNB; MRD19; NEDSDV; armadillo
• Gene ID: 1499
• mRNA Refseq: NM_001904.4
• Protein Refseq: NP_001895.1
• MIM: 116806
• UniProt ID: P35222