Active Recombinant Full Length Human DDX1 Protein, C-Flag-tagged

• Cat.No.: DDX1-356HFL
• Product Overview: Recombinant Full Length Human DDX1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein that acts as an ATP-dependent RNA helicase that has been found to promote coronaviruses replication.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: Biolayer interferometry (BLI) assay
• Molecular Mass: 82.3 kDa
• AA Sequence: MAAFSEMGVMPEIAQAVEEMDWLLP TDIQAESIPLILGGGDVLMAAETGS GKTGAFSIPVIQIVYETLKD QQEGKKGKTTIKTGASVLNKWQMNP YDRGSAFAIGSDGLCCQSREVKEWH GCRATKGLMKGKHYYEVSCH DQGLCRVGWSTMQASLDLGTDKFGF GFGGTGKKSHNKQFDNYGEEFTMHD TIGCYLDIDKGHVKFSKNGK DLGLAFEIPPHMKNQALFPACVLKN AELKFNFGEEEFKFPPKDGFVALSK APDGYIVKSQHSGNAQVTQT KFLPNAPKALIVEPSRELAEQTLNN IKQFKKYIDNPKLRELLIIGGVAAR DQLSVLENGVDIVVGTPGRL DDLVSTGKLNLSQVRFLVLDEADGL LSQGYSDFINRMHNQIPQVTSDGKR LQVIVCSATLHSFDVKKLSE KIMHFPTWVDLKGEDSVPDTVHHVV VPVNPKTDRLWERLGKSHIRTDDVH AKDNTRPGANSPEMWSEAIK ILKGEYAVRAIKEHKMDQAIIFCRT KIDCDNLEQYFIQQGGGPDKKGHQF SCVCLHGDRKPHERKQNLER FKKGDVRFLICTDVAARGIDIHGVP YVINVTLPDEKQNYVHRIGRVGRAE RMGLAISLVATEKEKVWYHV CSSRGKGCYNTRLKEDGGCTIWYNE MQLLSEIEEHLNCTISQVEPDIKVP VDEFDGKVTYGQKRAAGGGS
  YKGHVDILAPTVQELAALEKEAQTS FLHLGYLPNQLFRTFTRTRPLEQKL ISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome

Gene Information

• Gene Name: DDX1 DEAD-box helicase 1 [ Homo sapiens (human) ]
• Official Symbol: DDX1
• Synonyms: DBP-RB; UKVH5d
• Gene ID: 1653
• mRNA Refseq: NM_004939.3
• Protein Refseq: NP_004930.1
• MIM: 601257
• UniProt ID: Q92499