Active Recombinant Full Length Human EGLN2 Protein, C-Flag-tagged

• Cat.No.: EGLN2-174HFL
• Product Overview: Recombinant Full Length Human EGLN2 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The hypoxia inducible factor (HIF) is a transcriptional complex that is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. This gene encodes an enzyme responsible for this post-translational modification. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream RAB4B (RAB4B, member RAS oncogene family) gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: Enzyme activity (In vitro hydroxylation assay)
• Molecular Mass: 43.5 kDa
• AA Sequence: MDSPCQPQPLSQALPQLPGSSSEPL EPEPGRARMGVESYLPCPLLPSYHC PGVPSEASAGSGTPRATATS TTASPLRDGFGGQDGGELRPLQSEG AAALVTKGCQRLAAQGARPEAPKRK WAEDGGDAPSPSKRPWARQE NQEAEREGGMSCSCSSGSGEASAGL MEEALPSAPERLALDYIVPCMRYYG ICVKDSFLGAALGGRVLAEV EALKRGGRLRDGQLVSQRAIPPRSI RGDQIAWVEGHEPGCRSIGALMAHV DAVIRHCAGRLGSYVINGRT KAMVACYPGNGLGYVRHVDNPHGDG RCITCIYYLNQNWDVKVHGGLLQIF PEGRPVVANIEPLFDRLLIF
  WSDRRNPHEVKPAYATRYAITVWYF DAKERAAAKDKYQLASGQKGVQVPV SQPPTPTTRTRPLEQKLISEEDLAA NDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Pathways in cancer, Renal cell carcinoma

Gene Information

• Gene Name: EGLN2 egl-9 family hypoxia inducible factor 2 [ Homo sapiens (human) ]
• Official Symbol: EGLN2
• Synonyms: EIT6; PHD1; EIT-6; HPH-1; HPH-3; HIFPH1; HIF-PH1
• Gene ID: 112398
• mRNA Refseq: NM_053046.4
• Protein Refseq: NP_444274.1
• MIM: 606424
• UniProt ID: Q96KS0