Active Recombinant Full Length Human EIF2AK3 Protein, C-Flag-tagged

• Cat.No.: EIF2AK3-186HFL
• Product Overview: Recombinant Full Length Human EIF2AK3 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: Binding assay (ITC)
• Molecular Mass: 122.1 kDa
• AA Sequence: MERAISPGLLVRALLLLLLLLGLAA RTVAAGRARGLPAPTAEAAFGLGAA AAPTSATRVPAAGAVAAAEV TVEDAEALPAAAGEQEPRGPEPDDE TELRPRGRSLVIISTLDGRIAALDP ENHGKKQWDLDVGSGSLVSS SLSKPEVFGNKMIIPSLDGALFQWD RDRESMETVPFTVESLLESSYKFGD DVVLVGGKSLTTYGLSAYSG KVRYICSALGCRQWDSDEMEQEEDI LLLQRTQKTVRAVGPRSGNEKWNFS VGHFELRYIPDMETRAGFIE STFKPNENTEESKIISDVEEQEAAI MDIVIKVSVADWKVMAFSKKGGHLE WEYQFCTPIASAWLLKDGKV IPISLFDDTSYTSNDDVLEDEEDIV EAARGATENSVYLGMYRGQLYLQSS VRISEKFPSSPKALESVTNE NAIIPLPTIKWKPLIHSPSRTPVLV GSDEFDKCLSNDKFSHEEYSNGALS ILQYPYDNGYYLPYYKRERN KRSTQITVRFLDNPHYNKNIRKKDP VLLLHWWKEIVATILFCIIATTFIV RRLFHPHPHRQRKESETQCQ TENKYDSVSGEANDSSWNDIKNSGY ISRYLTDFEPIQCLGRGGFGVVFEA KNKVDDCNYAIKRIRLPNRE LAREKVMREVKALAKLEHPGIVRYF NAWLEAPPEKWQEKMDEIWLKDEST DWPLSSPSPMDAPSVKIRRM DPFSTKEHIEIIAPSPQRSRSFSVG ISCDQTSSSESQFSPLEFSGMDHED ISESVDAAYNLQDSCLTDCD VEDGTMDGNDEGHSFELCPSEASPY VRSRERTSSSIVFEDSGCDNASSKE EPKTNRLHIGNHCANKLTAF KPTSSKSSSEATLSISPPRPTTLSL DLTKNTTEKLQPSSPKVYLYIQMQL CRKENLKDWMNGRCTIEERE RSVCLHIFLQIAEAVEFLHSKGLMH RDLKPSNIFFTMDDVVKVGDFGLVT AMDQDEEEQTVLTPMPAYAR HTGQVGTKLYMSPEQIHGNSYSHKV DIFSLGLILFELLYPFSTQMERVRT LTDVRNLKFPPLFTQKYPCE YVMVQDMLSPSPMERPEAINIIENA VFEDLDFPGKTVLRQRSRSLSSSGT KHSRQSNNSHSPLPSNTRTRPLEQK LISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protein Kinase, Secreted Protein, Transmembrane
• Protein Pathways: Alzheimer's disease

Gene Information

• Gene Name: EIF2AK3 eukaryotic translation initiation factor 2 alpha kinase 3 [ Homo sapiens (human) ]
• Official Symbol: EIF2AK3
• Synonyms: PEK; WRS; PERK
• Gene ID: 9451
• mRNA Refseq: NM_004836.7
• Protein Refseq: NP_004827.4
• MIM: 604032
• UniProt ID: Q9NZJ5