Active Recombinant Full Length Human ERBB2 Protein, C-Flag-tagged

• Cat.No.: ERBB2-05HFL
• Product Overview: Recombinant Full Length Human ERBB2 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: ELISA capture for autoantibodies.
• Molecular Mass: 137.7 kDa
• AA Sequence: MELAALCRWGLLLALLPPGAASTQV CTGTDMKLRLPASPETHLDMLRHLY QGCQVVQGNLELTYLPTNAS LSFLQDIQEVQGYVLIAHNQVRQVP LQRLRIVRGTQLFEDNYALAVLDNG DPLNNTTPVTGASPGGLREL QLRSLTEILKGGVLIQRNPQLCYQD TILWKDIFHKNNQLALTLIDTNRSR ACHPCSPMCKGSRCWGESSE DCQSLTRTVCAGGCARCKGPLPTDC CHEQCAAGCTGPKHSDCLACLHFNH SGICELHCPALVTYNTDTFE SMPNPEGRYTFGASCVTACPYNYLS TDVGSCTLVCPLHNQEVTAEDGTQR CEKCSKPCARVCYGLGMEHL REVRAVTSANIQEFAGCKKIFGSLA FLPESFDGDPASNTAPLQPEQLQVF ETLEEITGYLYISAWPDSLP DLSVFQNLQVIRGRILHNGAYSLTL QGLGISWLGLRSLRELGSGLALIHH NTHLCFVHTVPWDQLFRNPH QALLHTANRPEDECVGEGLACHQLC ARGHCWGPGPTQCVNCSQFLRGQEC VEECRVLQGLPREYVNARHC LPCHPECQPQNGSVTCFGLEADQCV ACAHYKDPPFCVARCPSGVKPDLSY MPIWKFPDEEGACQPCPINC THSCVDLDDKGCPAEQRASPLTSII SAVVGILLVVVLGVVFGILIKRRQQ KIRKYTMRRLLQETELVEPL TPSGAMPNQAQMRILKETELRKVKV LGSGAFGTVYKGIWIPDGENVKIPV AIKVLRENTSPKANKEILDE AYVMAGVGSPYVSRLLGICLTSTVQ LVTQLMPYGCLLDHVRENRGRLGSQ DLLNWCMQIAKGMSYLEDVR LVHRDLAARNVLVKSPNHVKITDFG LARLLDIDETEYHADGGKVPIKWMA LESILRRRFTHQSDVWSYGV TVWELMTFGAKPYDGIPAREIPDLL EKGERLPQPPICTIDVYMIMVKCWM IDSECRPRFRELVSEFSRMA RDPQRFVVIQNEDLGPASPLDSTFY RSLLEDDDMGDLVDAEEYLVPQQGF FCPDPAPGAGGMVHHRHRSS STRSGGGDLTLGLEPSEEEAPRSPL APSEGAGSDVFDGDLGMGAAKGLQS LPTHDPSPLQRYSEDPTVPL PSETDGYVAPLTCSPQPEYVNQPDV RPQPPSPREGPLPAARPAGATLERP KTLSPGKNGVVKDVFAFGGA VENPEYLTPQGGAAPQPHPPPAFSP AFDNLYYWDQDPPERGAPPSTFKGT PTAENPEYLGLDVPVTRTRPLEQKL ISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: Adherens junction, Bladder cancer, Calcium signaling pathway, Endometrial cancer, ErbB signaling pathway, Focal adhesion, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer

Gene Information

• Gene Name: ERBB2 erb-b2 receptor tyrosine kinase 2 [ Homo sapiens (human) ]
• Official Symbol: ERBB2
• Synonyms: NEU; NGL; HER2; TKR1; CD340; HER-2; VSCN2; MLN 19; MLN-19; c-ERB2; c-ERB-2; HER-2/neu; p185(erbB2)
• Gene ID: 2064
• mRNA Refseq: NM_004448.4
• Protein Refseq: NP_004439.2
• MIM: 164870
• UniProt ID: P04626

Reviews

05/09/2022

Worked as expected, satisfied.

03/16/2022

Reliable in protein assays.

09/27/2020

Quick and reliable shipping.

Q&As

Do you have a recommended protocol for immobilizing any avi-tagged recombinant proteins on a streptavidin coated plate?

1.Prepare the streptavidin-coated plate by washing it with PBS (phosphate-buffered saline) or another suitable buffer to remove any impurities. 2.Dilute the Avi-tagged protein in the desired buffer at the desired concentration. It is important to use a buffer that is compatible with the protein and does not interfere with its activity or stability. 3.Add the diluted protein to the streptavidin-coated plate and incubate it for a suitable amount of time at room temperature or 4°C. The exact conditions will depend on the specific protein and the desired level of immobilization. 4.Wash the plate with a suitable buffer to remove any unbound protein. 5.Block the remaining binding sites on the plate with a suitable blocking agent such as BSA (bovine serum albumin) or casein. 6.Wash the plate again to remove any unbound blocking agent. 7.Use the immobilized protein for further experiments such as ELISA, Western blotting, or other assays.

What is the primary role of ERBB2 in cell signaling?

ERBB2 is a receptor tyrosine kinase involved in the regulation of cell growth and differentiation.

What therapeutic agents target ERBB2 in cancer treatment?

Therapeutic agents targeting ERBB2 include trastuzumab and lapatinib.

In which types of cancer is ERBB2 commonly overexpressed?

ERBB2 is commonly overexpressed in breast, ovarian, and stomach cancers.

How does ERBB2 overexpression affect patient prognosis in cancer?

ERBB2 overexpression is often associated with aggressive disease and poor prognosis.

How can ERBB2 levels be assessed in a clinical setting?

ERBB2 levels can be assessed using immunohistochemistry and fluorescence in situ hybridization (FISH) in tissue samples.

How is ERBB2 involved in cell growth and proliferation?

It forms heterodimers with other ERBB receptors, activating pathways that promote cell growth and survival.

Are there known genetic mutations that lead to ERBB2 overexpression?

Amplification of the ERBB2 gene leads to its overexpression in cancer cells.