Active Recombinant Full Length Human ERBB4 Protein, C-Flag-tagged

• Cat.No.: ERBB4-513HFL
• Product Overview: Recombinant Full Length Human ERBB4 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene is a member of the Tyr protein kinase family and the epidermal growth factor receptor subfamily. It encodes a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. The protein binds to and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. Multiple proteolytic events allow for the release of a cytoplasmic fragment and an extracellular fragment. Mutations in this gene have been associated with cancer. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: ErbB4 activity verified in a biochemical assay : ErbB4 (v-erb-a erythroblastic leukemia viral oncogene homolog 4) activity was measured in a homogeneous time-resolved fluorescent (HTRF®) assay. ErbB4 is a tyrosine protein kinase and a member of the epidermal growth factor receptor subfamily. Varying concentrations of ErbB4 were added to a reaction mix containing ATP and a biotinylated kinase substrate and the reaction mixture was incubated to allow the protein to phosphorylate the tyrosine residue in the substrate. HTRF detection reagents were then added, and the time-resolved fluorescent signal was measured on a Flexstation 3 microplate reader. The time resolved fluorescent signal is expressed as “delta R” or “ΔR” and is a ratio calculated from the fluorescent emission intensities of the donor and acceptor fluors.
• Molecular Mass: 142.6 kDa
• AA Sequence: MKPATGLWVWVSLLVAAGTVQPSDS QSVCAGTENKLSSLSDLEQQYRALR KYYENCEVVMGNLEITSIEH NRDLSFLRSVREVTGYVLVALNQFR YLPLENLRIIRGTKLYEDRYALAIF LNYRKDGNFGLQELGLKNLT EILNGGVYVDQNKFLCYADTIHWQD IVRNPWPSNLTLVSTNGSSGCGRCH KSCTGRCWGPTENHCQTLTR TVCAEQCDGRCYGPYVSDCCHRECA GGCSGPKDTDCFACMNFNDSGACVT QCPQTFVYNPTTFQLEHNFN AKYTYGAFCVKKCPHNFVVDSSSCV RACPSSKMEVEENGIKMCKPCTDIC PKACDGIGTGSLMSAQTVDS SNIDKFINCTKINGNLIFLVTGIHG DPYNAIEAIDPEKLNVFRTVREITG FLNIQSWPPNMTDFSVFSNL VTIGGRVLYSGLSLLILKQQGITSL QFQSLKEISAGNIYITDNSNLCYYH TINWTTLFSTINQRIVIRDN RKAENCTAEGMVCNHLCSSDGCWGP GPDQCLSCRRFSRGRICIESCNLYD GEFREFENGSICVECDPQCE KMEDGLLTCHGPGPDNCTKCSHFKD GPNCVEKCPDGLQGANSFIFKYADP DRECHPCHPNCTQGCNGPTS HDCIYYPWTGHSTLPQHARTPLIAA GVIGGLFILVIVGLTFAVYVRRKSI KKKRALRRFLETELVEPLTP SGTAPNQAQLRILKETELKRVKVLG SGAFGTVYKGIWVPEGETVKIPVAI KILNETTGPKANVEFMDEAL IMASMDHPHLVRLLGVCLSPTIQLV TQLMPHGCLLEYVHEHKDNIGSQLL LNWCVQIAKGMMYLEERRLV HRDLAARNVLVKSPNHVKITDFGLA RLLEGDEKEYNADGGKMPIKWMALE CIHYRKFTHQSDVWSYGVTI WELMTFGGKPYDGIPTREIPDLLEK GERLPQPPICTIDVYMVMVKCWMID ADSRPKFKELAAEFSRMARD PQRYLVIQGDDRMKLPSPNDSKFFQ NLLDEEDLEDMMDAEEYLVPQAFNI PPPIYTSRARIDSNRNQFVY RDGGFAAEQGVSVPYRAPTSTIPEA PVAQGATAEIFDDSCCNGTLRKPVA PHVQEDSSTQRYSADPTVFA PERSPRGELDEEGYMTPMRDKPKQE YLNPVEENPFVSRRKNGDLQALDNP EYHNASNGPPKAEDEYVNEP LYLNTFANTLGKAEYLKNNILSMPE KAKKAFDNPDYWNHSLPPRSTLQHP DYLQEYSTKYFYKQNGRIRP
  IVAENPEYLSEFSLKPGTVLPPPPY RHRNTVVTRTRPLEQKLISEEDLAA NDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: Calcium signaling pathway, Endocytosis, ErbB signaling pathway

Gene Information

• Gene Name: ERBB4 erb-b2 receptor tyrosine kinase 4 [ Homo sapiens (human) ]
• Official Symbol: ERBB4
• Synonyms: HER4; ALS19; p180erbB4
• Gene ID: 2066
• mRNA Refseq: NM_001042599.1
• Protein Refseq: NP_001036064.1
• MIM: 600543
• UniProt ID: Q15303