Active Recombinant Full Length Human GPNMB Protein, C-Flag-tagged

• Cat.No.: GPNMB-321HFL
• Product Overview: Recombinant Full Length Human GPNMB Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: Pull-down assay
• Molecular Mass: 61.5 kDa
• AA Sequence: MECLYYFLGFLLLAARLPLDAAKRF HDVLGNERPSAYMREHNQLNGWSSD ENDWNEKLYPVWKRGDMRWK NSWKGGRVQAVLTSDSPALVGSNIT FAVNLIFPRCQKEDANGNIVYEKNC RNEAGLSADPYVYNWTAWSE DSDGENGTGQSHHNVFPDGKPFPHH PGWRRWNFIYVFHTLGQYFQKLGRC SVRVSVNTANVTLGPQLMEV TVYRRHGRAYVPIAQVKDVYVVTDQ IPVFVTMFQKNDRNSSDETFLKDLP IMFDVLIHDPSHFLNYSTIN YKWSFGDNTGLFVSTNHTVNHTYVL NGTFSLNLTVKAAAPGPCPPPPPPP RPSKPTPSLATTLKSYDSNT PGPAGDNPLELSRIPDENCQINRYG HFQATITIVEGILEVNIIQMTDVLM PVPWPESSLIDFVVTCQGSI PTEVCTIISDPTCEITQNTVCSPVD VDEMCLLTVRRTFNGSGTYCVNLTL GDDTSLALTSTLISVPDRDP ASPLRMANSALISVGCLAIFVTVIS LLVYKKHKEYNPIENSPGNVVRSKG LSVFLNRAKAVFFPGNQEKD
  PLLKNQEFKGVSTRTRPLEQKLISE EDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Transmembrane

Gene Information

• Gene Name: GPNMB glycoprotein nmb [ Homo sapiens (human) ]
• Official Symbol: GPNMB
• Synonyms: NMB; HGFIN; PLCA3
• Gene ID: 10457
• mRNA Refseq: NM_001005340.2
• Protein Refseq: NP_001005340.1
• MIM: 604368
• UniProt ID: Q14956