Active Recombinant Full Length Human GRK6 Protein

• Cat.No.: GRK6-2446HF
• Product Overview: Full-length human GPRK6 [1-560(end) amino acids of accession number NP_001004105.1] was expressed as N-terminal GST-fusion protein using baculovirus expression system. GST-GPRK6 was purified by using glutathione sepharose chromatography. GST-GPRK6 was cleaved by PreScission protease and GST-free GPRK6(65 kDa) was collected as flow-through fraction from glutathione sepharose chromatography.

Specification

• Source: Insect (sf21)
• Species: Human
• Tag: Tag-free
• Form: 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
• Bio-activity: The activity was measured by off-chip mobility shift assay(MSA). The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by MSA device.
• Protein length: 1-560
• Purity: 96%
• Applications: SDS-PAGE
• Storage: Store at -80C. Avoid repeating freeze-thaws.

Gene Information

• Gene Name: G protein-coupled receptor kinase 6
• Official Symbol: GRK6
• Synonyms: GRK6;GPRK6
• Protein Refseq: NP_001004105.2
• UniProt ID: P43250