Active Recombinant Full Length Human HDAC4 Protein, C-Flag-tagged

• Cat.No.: HDAC4-256HFL
• Product Overview: Recombinant Full Length Human HDAC4 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: Enzyme substrate
• Molecular Mass: 118.9 kDa
• AA Sequence: MSSQSHPDGLSGRDQPVELLNPARV NHMPSTVDVATALPLQVAPSAVPMD LRLDHQFSLPVAEPALREQQ LQQELLALKQKQQIQRQILIAEFQR QHEQLSRQHEAQLHEHIKQQQEMLA MKHQQELLEHQRKLERHRQE QELEKQHREQKLQQLKNKEKGKESA VASTEVKMKLQEFVLNKKKALAHRN LNHCISSDPRYWYGKTQHSS LDQSSPPQSGVSTSYNHPVLGMYDA KDDFPLRKTASEPNLKLRSRLKQKV AERRSSPLLRRKDGPVVTAL KKRPLDVTDSACSSAPGSGPSSPNN SSGSVSAENGIAPAVPSIPAETSLA HRLVAREGSAAPLPLYTSPS LPNITLGLPATGPSAGTAGQQDAER LTLPALQQRLSLFPGTHLTPYLSTS PLERDGGAAHSPLLQHMVLL EQPPAQAPLVTGLGALPLHAQSLVG ADRVSPSIHKLRQHRPLGRTQSAPL PQNAQALQHLVIQQQHQQFL EKHKQQFQQQQLQMNKIIPKPSEPA RQPESHPEETEEELREHQALLDEPY LDRLPGQKEAHAQAGVQVKQ EPIESDEEEAEPPREVEPGQRQPSE QELLFRQQALLLEQQRIHQLRNYQA SMEAAGIPVSFGGHRPLSRA QSSPASATFPVSVQEPPTKPRFTTG LVYDTLMLKHQCTCGSSSSHPEHAG RIQSIWSRLQETGLRGKCEC IRGRKATLEELQTVHSEAHTLLYGT NPLNRQKLDSKKLLGSLASVFVRLP CGGVGVDSDTIWNEVHSAGA ARLAVGCVVELVFKVATGELKNGFA VVRPPGHHAEESTPMGFCYFNSVAV AAKLLQQRLSVSKILIVDWD VHHGNGTQQAFYSDPSVLYMSLHRY DDGNFFPGSGAPDEVGTGPGVGFNV NMAFTGGLDPPMGDAEYLAA FRTVVMPIASEFAPDVVLVSSGFDA VEGHPTPLGGYNLSARCFGYLTKQL MGLAGGRIVLALEGGHDLTA ICDASEACVSALLGNELDPLPEKVL QQRPNANAVRSMEKVMEIHSKYWRC LQRTTSTAGRSLIEAQTCEN
  EEAETVTAMASLSVGVKPAEKRPDE EPMEEEPPLTRTRPLEQKLISEEDL AANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Transcription Factors

Gene Information

• Gene Name: HDAC4 histone deacetylase 4 [ Homo sapiens (human) ]
• Official Symbol: HDAC4
• Synonyms: HD4; AHO3; BDMR; HDACA; HA6116; HDAC-4; HDAC-A; NEDCHF; NEDCHID
• Gene ID: 9759
• mRNA Refseq: NM_006037.4
• Protein Refseq: NP_006028.2
• MIM: 605314
• UniProt ID: P56524