| Species : |
Human |
| Source : |
Insect Cells |
| Tag : |
Flag |
| Protein Length : |
1-353 aa |
| Description : |
This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. This gene has been described to generate two alternatively spliced transcript variants which encode different isoforms. |
| Form : |
Recombinant hnRNPA2B1-B1 protein is supplied at a concentration of 0.1 ug/ul in 25 mM HEPES pH 7.5, 500mM NaCl, 5% glycerol, 0.04% TritonX-100, 0.2 mM TCEP. |
| Molecular Mass : |
38.4 kDa |
| Purity : |
> 90% |
| Applications : |
Recombinant hnRNPA2B1-B1 protein is suitable for use in evaluation of protein-protein interactions and RNA binding assays.
Activity Assay Details: 75 pmol biotinylated m6A ssDNA oligos (5'-GTTGCCTGTTCGTGTTGG/m6A/ CTTGCCTGT-3') were incubated with hnRNPA2B1 protein in binding buffer including 25 mM Tris-HCl pH 7.4, 150 mM NaCl, 5% glycerol, 0.1% Triton X-100 for 3 hr at 4 centigrade. The DNA-hnRNPA2B1 compounds were incubated with streptavidin beads for 1 hr at 4 centigrade and pull-down complexes were run on SDS-PAGE gel, stained with Coomassie Blue. Biotinylated, unmethylated ssDNA oligos used as a negative control. |
| Storage : |
Recombinant proteins in solution are temperature sensitive and must be stored at -80 centigrade to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| Concentration : |
0.1 μg/μL |
