Active Recombinant Full Length Human KYAT1 Protein, C-Flag-tagged
Cat.No. : | KYAT1-366HFL |
Product Overview : | Recombinant Full Length Human KYAT1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells. |
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Description : | This gene encodes a cytosolic enzyme that is responsible for the metabolism of cysteine conjugates of certain halogenated alkenes and alkanes. This metabolism can form reactive metabolites leading to nephrotoxicity and neurotoxicity. Increased levels of this enzyme have been linked to schizophrenia. Multiple transcript variants that encode different isoforms have been identified for this gene. |
Source : | Mammalian cells |
Species : | Human |
Tag : | Flag |
Form : | 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol. |
Bio-activity : | The specific activity of KATI was determined by measuring the product Kynurenic acid formation from a conversion of Kynurenine. The reaction was carried out at 37? for 15min in the buffer containing 100 mM PBS, pH7.4, 2 mM a-oxoglutarate, 40µM PLP (pyridoxal 5'-phosphate), and 0.5 mM kynurenine as the substrate. |
Molecular Mass : | 47.7 kDa |
AA Sequence : | MAKQLQARRLDGIDYNPWVEFVKLA SEHDVVNLGQGFPDFPPPDFAVEAF QHAVSGDFMLNQYTKTFGYP PLTKILASFFGELLGQEIDPLRNVL VTVGGYGALFTAFQALVDEGDEVII IEPFFDCYEPMTMMAGGRPV FVSLKPGPIQNGELGSSSNWQLDPM ELAGKFTSRTKALVLNTPNNPLGKV FSREELELVASLCQQHDVVC ITDEVYQWMVYDGHQHISIASLPGM WERTLTIGSAGKTFSATGWKVGWVL GPDHIMKHLRTVHQNSVFHC PTQSQAAVAESFEREQLLFRQPSSY FVQFPQAMQRCRDHMIRSLQSVGLK PIIPQGSYFLITDISDFKRK MPDLPGAVDEPYDRRFVKWMIKNKG LVAIPVSIFYSVPHQKHFDHYIRFC FVKDEATLQAMDEKLRKWKV ELTRTRPLEQKLISEEDLAANDILD YKDDDDKV |
Purity : | > 80% as determined by SDS-PAGE and Coomassie blue staining. |
Stability : | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
Storage : | Store at -80 centigrade. |
Concentration : | >50 ug/mL as determined by microplate BCA method. |
Preparation : | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
Gene Name : | KYAT1 kynurenine aminotransferase 1 [ Homo sapiens (human) ] |
Official Symbol : | KYAT1 |
Synonyms : | GTK; KAT1; KATI; CCBL1 |
Gene ID : | 883 |
mRNA Refseq : | NM_004059.5 |
Protein Refseq : | NP_004050.3 |
MIM : | 600547 |
UniProt ID : | Q16773 |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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