Active Recombinant Full Length Human LCK Protein, DDK-tagged, Biotinylated

• Cat.No.: LCK-8482HF
• Product Overview: Full-length human LCK [1-509(end) amino acids of accession number NP_005347.2] was expressed as N- terminal DYKDDDDK tagged, biotinylated protein (62 kDa) using baculovirus expression system. The protein was purified by using DYKDDDDK tag antibody agarose.

Specification

• Source: Insect (sf21)
• Species: Human
• Tag: DDK
• Form: 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
• Bio-activity: The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
• Protein length: 1-509(end)
• Purity: 99%
• Applications: SDS-PAGE
• Storage: Store at -80C. Avoid repeating freeze-thaws.

Gene Information

• Gene Name: lymphocyte-specific protein tyrosine kinase
• Official Symbol: LCK
• Synonyms: LCK;IMD22;LSK;YT16;p56lck;pp58lck
• Protein Refseq: NP_005347.2
• UniProt ID: P06239

Reviews

04/24/2022

Good stability, long shelf-life.

10/29/2020

Consistent quality across batches.

03/11/2019

Pure product, reliable results.

Q&As

What role does LCK play in the development and maturation of T cells in the thymus?

LCK plays a vital role in the development and maturation of T cells in the thymus, especially during the processes of positive and negative selection, ensuring the generation of a functional and self-tolerant T-cell repertoire.

Can LCK activity be modulated by pharmacological agents?

Yes, LCK activity can be modulated by several pharmacological agents, including kinase inhibitors like dasatinib, which have been explored for therapeutic purposes in various diseases.

How is LCK activation regulated post-translationally?

LCK activation is regulated post-translationally through phosphorylation at specific tyrosine residues, with its activity being modulated by both activating and inhibitory phosphorylations.

What is the primary function of LCK in T-cell signaling?

LCK is a pivotal Src family kinase that plays a crucial role in initiating T-cell receptor (TCR) signaling by phosphorylating key tyrosines on the CD3 and ζ-chains of the TCR complex.

How does LCK interact with other kinases in T-cell signaling pathways?

LCK interacts with other kinases such as ZAP-70 and Fyn in T-cell signaling pathways, orchestrating a cascade of phosphorylation events that lead to T-cell activation.

Are there any known diseases or conditions associated with LCK dysregulation?

Dysregulation of LCK has been associated with various conditions, including autoimmune diseases, immunodeficiencies, and certain leukemias.

How does LCK contribute to T-cell receptor (TCR) complex formation and activation?

LCK contributes to TCR complex formation and activation by phosphorylating the immunoreceptor tyrosine-based activation motifs (ITAMs) on the TCR, facilitating the recruitment and activation of ZAP-70 kinase.