Active Recombinant Full Length Human LIG1 Protein, C-Flag-tagged

• Cat.No.: LIG1-196HFL
• Product Overview: Recombinant Full Length Human LIG1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the ATP-dependent DNA ligase protein family. The encoded protein functions in DNA replication, recombination, and the base excision repair process. Mutations in this gene that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents. Disruption of this gene may also be associated with a variety of cancers. Alternative splicing results in multiple transcript variants.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: Enzyme activity
• Molecular Mass: 101.6 kDa
• AA Sequence: MQRSIMSFFHPKKEGKAKKPEKEAS NSSRETEPPPKAALKEWNGVVSESD SPVKRPGRKAARVLGSEGEE EDEALSPAKGQKPALDCSQVSPPRP ATSPENNASLSDTSPMDSSPSGIPK RRTARKQLPKRTIQEVLEEQ SEDEDREAKRKKEEEEEETPKESLT EAEVATEKEGEDGDQPTTPPKPLKT SKAETPTESVSEPEVATKQE LQEEEEQTKPPRRAPKTLSSFFTPR KPAVKKEVKEEEPGAPGKEGAAEGP LDPSGYNPAKNNYHPVEDAC WKPGQKVPYLAVARTFEKIEEVSAR LRMVETLSNLLRSVVALSPPDLLPV LYLSLNHLGPPQQGLELGVG DGVLLKAVAQATGRQLESVRAEAAE KGDVGLVAENSRSTQRLMLPPPPLT ASGVFSKFRDIARLTGSAST AKKIDIIKGLFVACRHSEARFIARS LSGRLRLGLAEQSVLAALSQAVSLT PPGQEFPPAMVDAGKGKTAE ARKTWLEEQGMILKQTFCEVPDLDR IIPVLLEHGLERLPEHCKLSPGIPL KPMLAHPTRGISEVLKRFEE AAFTCEYKYDGQRAQIHALEGGEVK IFSRNQEDNTGKYPDIISRIPKIKL PSVTSFILDTEAVAWDREKK QIQPFQVLTTRKRKEVDASEIQVQV CLYAFDLIYLNGESLVREPLSRRRQ LLRENFVETEGEFVFATSLD TKDIEQIAEFLEQSVKDSCEGLMVK TLDVDATYEIAKRSHNWLKLKKDYL DGVGDTLDLVVIGAYLGRGK RAGRYGGFLLASYDEDSEELQAICK LGTGFSDEELEEHHQSLKALVLPSP RPYVRIDGAVIPDHWLDPSA VWEVKCADLSLSPIYPAARGLVDSD KGISLRFPRFIRVREDKQPEQATTS AQVACLYRKQSQIQNQQGED
  SGSDPEDTYTRTRPLEQKLISEEDL AANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Base excision repair, DNA replication, Mismatch repair, Nucleotide excision repair

Gene Information

• Gene Name: LIG1 DNA ligase 1 [ Homo sapiens (human) ]
• Official Symbol: LIG1
• Synonyms: LIGI; IMD96; hLig1
• Gene ID: 3978
• mRNA Refseq: NM_000234.3
• Protein Refseq: NP_000225.1
• MIM: 126391
• UniProt ID: P18858