Active Recombinant Full Length Human myeloperoxidase Protein, His tagged

• Cat.No.: MPO-10HFL
• Product Overview: Recombinant Full Length Human MPO Protein with a C-terminal 10-His tag was expressed in Mouse myeloma cell line.

Specification

• Species: Human
• Source: NS0
• Tag: His
• Protein Length: 1-745aa
• Description: Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils.
• Tag: C-10×His
• Bio-activity: Measured by its ability to oxidize guaiacol in the presence of hydrogen peroxide. The specific activity is > 50,000 pmol/min/μg, as measured under the described conditions.
• Molecular Mass: 80 kDa
• N-terminal Sequence Analysis: Ala49
• Endotoxin: < 1 EU/μg by LAL
• Purity: > 90% by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  6 months from date of receipt, -20 to -70 centigrade as supplied.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in Tris and NaCl.
• Reconstitution: Reconstitute at 0.5-1 mg/mL in sterile, deionized water.
• Shipping: The product is shipped with polar packs.
• References: 1. Klebanoff, S.J. (2005) J. Leukoc. Biol. 77:598.
  2. Hansson, M. et al. (2006) Arch. Biochem. Biophys. 445:214.
  3. Hashinaka, K. et al. (1988) Biochemistry 27:5906.

Gene Information

• Gene Name: MPO myeloperoxidase [ Homo sapiens (human) ]
• Official Symbol: MPO
• Synonyms: MPO; myeloperoxidase
• Gene ID: 4353
• mRNA Refseq: NM_000250
• Protein Refseq: NP_000241
• MIM: 606989
• UniProt ID: P05164