Active Recombinant Full Length Human MYLK2 Protein, GST-tagged

• Cat.No.: MYLK2-8782HF
• Product Overview: Full-length human skMLCK [1-596(end) amino acids of accession number NP_149109.1] was expressed as N-terminal GST-fusion protein (93 kDa) using baculovirus expression system. GST-skMLCK was purified by using glutathione sepharose chromatography.

Specification

• Source: Insect (sf21)
• Species: Human
• Tag: GST
• Form: 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
• Bio-activity: The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorecence-labeled substrate, Mg(or Mn)/ATP, and Ca/Calmodulin . The phosphorylated and unphosphorylated substrates were separated and detected by LabChip ™3000.
• Protein length: 1-596
• Purity: 67%
• Applications: SDS-PAGE
• Storage: Store at -80C. Avoid repeating freeze-thaws.

Gene Information

• Gene Name: myosin light chain kinase 2
• Official Symbol: MYLK2
• Synonyms: MYLK2;KMLC;MLCK;MLCK2;skMLCK
• Protein Refseq: NP_149109.1
• UniProt ID: Q9H1R3