Active Recombinant Full Length Human PIK3CA Protein, DDK-tagged, Biotinylated

• Cat.No.: PIK3CA-8555HF
• Product Overview: Full-length human PIK3CA[1-1068(end) amino acids and H1047R of accession number NP_006209.2] was co-expressed as N-terminal DYKDDDDK tagged, biotinylated protein (128 kDa) with PIK3R1[1- 724(end) amino acids(84kDa) of accession number NP_852664.1] using baculovirus expression system. The protein was purified by using DYKDDDDK tag antibody agarose.

Specification

• Source: Insect (sf21)
• Species: Human
• Tag: Flag
• Form: 50 mM Tris-HCl, 150 mM NaCl, 0.01% Brij35, 1 mM DTT, 10% glycerol, pH7.5
• Bio-activity: The activity was determined by ADP-Glo™ Assay. The enzyme was incubated with Lipid substrate and Mg(or Mn)/ATP. The phosphorylation was detected by the ADP-Glo™ Kinase Assay technology (luminescent ADP detection assay).
• Protein length: 1-1068(end)
• Purity: 81%
• Applications: SDS-PAGE
• Storage: Store at -80C. Avoid repeating freeze-thaws.

Gene Information

• Gene Name: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha
• Official Symbol: PIK3CA
• Synonyms: PIK3CA;CLAPO;CLOVE;CWS5;MCAP;MCM;MCMTC;PI3K;PI3K-alpha;p110-alpha
• Protein Refseq: NP_006209.2
• UniProt ID: P42336