Active Recombinant Full Length Human PPM1H Protein, C-Flag-tagged

• Cat.No.: PPM1H-456HFL
• Product Overview: Recombinant Full Length Human PPM1H Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Dephosphorylates CDKN1B at 'Thr-187', thus removing a signal for proteasomal degradation.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: In vitro phosphatase assay
• Molecular Mass: 56.3 kDa
• AA Sequence: MLTRVKSAVANFMGGIMAGSSGSEH GGGSCGGSDLPLRFPYGRPEFLGLS QDEVECSADHIARPILILKE TRRLPWATGYAEVINAGKSTHNEDQ ASCEVLTVKKKAGAVTSTPNRNSSK RRSSLPNGEGLQLKENSESE GVSCHYWSLFDGHAGSGAAVVASRL LQHHITEQLQDIVDILKNSAVLPPT CLGEEPENTPANSRTLTRAA SLRGGVGAPGSPSTPPTRFFTEKKI PHECLVIGALESAFKEMDLQIERER SSYNISGGCTALIVICLLGK LYVANAGDSRAIIIRNGEIIPMSSE FTPETERQRLQYLAFMQPHLLGNEF THLEFPRRVQRKELGKKMLY RDFNMTGWAYKTIEDEDLKFPLIYG EGKKARVMATIGVTRGLGDHDLKVH DSNIYIKPFLSSAPEVRIYD LSKYDHGSDDVLILATDGLWDVLSN EEVAEAITQFLPNCDPDDPHRYTLA AQDLVMRARGVLKDRGWRIS
  NDRLGSGDDISVYVIPLIHGNKLST RTRPLEQKLISEEDLAANDILDYKD DDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Phosphatase

Gene Information

• Gene Name: PPM1H protein phosphatase, Mg2+/Mn2+ dependent 1H [ Homo sapiens (human) ]
• Official Symbol: PPM1H
• Synonyms: URCC2; ARHCL1; NERPP-2C
• Gene ID: 57460
• mRNA Refseq: NM_020700.2
• Protein Refseq: NP_065751.1
• MIM: 616016
• UniProt ID: Q9ULR3