Active Recombinant Full Length Human RELA Protein, C-Flag-tagged
Cat.No. : | RELA-11HFL |
Product Overview : | Recombinant Full Length Human RELA Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells. |
- Specification
- Gene Information
- Related Products
Description : | NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. |
Source : | Mammalian cells |
Species : | Human |
Tag : | Flag |
Form : | 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol. |
Bio-activity : | RELA Activity Verified in a DNA-binding Assay: RELA activity was measured in a colorimetric DNA-binding assay. Double-stranded oligonucleotide containing the RELA consensus DNA-binding sequence was incubated with dilutions of the purified RELA protein. RELA bound to the oligo was captured onto the surface of a microtiter plate and after washing, bound RELA was detected with an anti-RELA primary antibody followed by an HRP-labeled secondary antibody. After initial color development, the reaction was quenched and the color intensity was measured at 450nm. ELISA binding assay WB positive control EMSA assay Binding assay Pull-down assay |
Molecular Mass : | 60 kDa |
AA Sequence : | MDELFPLIFPAEPAQASGPYVEIIE QPKQRGMRFRYKCEGRSAGSIPGER STDTTKTHPTIKINGYTGPG TVRISLVTKDPPHRPHPHELVGKDC RDGFYEAELCPDRCIHSFQNLGIQC VKKRDLEQAISQRIQTNNNP FQVPIEEQRGDYDLNAVRLCFQVTV RDPSGRPLRLPPVLSHPIFDNRAPN TAELKICRVNRNSGSCLGGD EIFLLCDKVQKEDIEVYFTGPGWEA RGSFSQADVHRQVAIVFRTPPYADP SLQAPVRVSMQLRRPSDREL SEPMEFQYLPDTDDRHRIEEKRKRT YETFKSIMKKSPFSGPTDPRPPPRR IAVPSRSSASVPKPAPQPYP FTSSLSTINYDEFPTMVFPSGQISQ ASALAPAPPQVLPQAPAPAPAPAMV SALAQAPAPVPVLAPGPPQA VAPPAPKPTQAGEGTLSEALLQLQF DDEDLGALLGNSTDPAVFTDLASVD NSEFQQLLNQGIPVAPHTTE PMLMEYPEAITRLVTGAQRPPDPAP APLGAPGLPNGLLSGDEDFSSIADM DFSALLSQISSTRTRPLEQKLISEE DLAANDILDYKDDDDKV |
Purity : | > 80% as determined by SDS-PAGE and Coomassie blue staining. |
Stability : | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
Storage : | Store at -80 centigrade. |
Concentration : | >50 ug/mL as determined by microplate BCA method. |
Preparation : | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
Protein Families : | Druggable Genome, Transcription Factors |
Protein Pathways : | Acute myeloid leukemia, Adipocytokine signaling pathway, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Cytosolic DNA-sensing pathway, Epithelial cell signaling in Helicobacter pylori infection, MAPK signaling pathway, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, Pancreatic cancer, Pathways in cancer, Prostate cancer, RIG-I-like receptor signaling pathway, Small cell lung cancer, T cell receptor signaling pathway, Toll-like receptor signaling pathway |
Gene Name : | RELA RELA proto-oncogene, NF-kB subunit [ Homo sapiens (human) ] |
Official Symbol : | RELA |
Synonyms : | p65; CMCU; NFKB3 |
Gene ID : | 5970 |
mRNA Refseq : | NM_021975.4 |
Protein Refseq : | NP_068810.3 |
MIM : | 164014 |
UniProt ID : | Q04206 |
Products Types
◆ Recombinant Protein | ||
RELA-333H | Recombinant Human NFκB3 (RELA/p65) Protein, Flag-tagged | +Inquiry |
Rela-5456M | Recombinant Mouse Rela Protein, Myc/DDK-tagged | +Inquiry |
RELA-1878H | Recombinant Human RELA Protein, His (Fc)-Avi-tagged | +Inquiry |
rela-2736Z | Recombinant Zebrafish rela Protein, His-tagged | +Inquiry |
RELA-627TH | Recombinant Human RELA, C-Terminal Truncated | +Inquiry |
◆ Lysates | ||
RELA-2423HCL | Recombinant Human RELA 293 Cell Lysate | +Inquiry |
◆ Assay kits | ||
Kit-1652 | HEK 293 RELA Nuclear Translocation Assay Kit | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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