Active Recombinant Full Length Human RNH1 Protein, C-Flag-tagged

• Cat.No.: RNH1-447HFL
• Product Overview: Recombinant Full Length Human RNH1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Placental ribonuclease inhibitor (PRI) is a member of a family of proteinaceous cytoplasmic RNase inhibitors that occur in many tissues and bind to both intracellular and extracellular RNases. In addition to control of intracellular RNases, the inhibitor may have a role in the regulation of angiogenin. Ribonuclease inhibitor, of 50,000 Da, binds to ribonucleases and holds them in a latent form. Since neutral and alkaline ribonucleases probably play a critical role in the turnover of RNA in eukaryotic cells, RNH may be essential for control of mRNA turnover; the interaction of eukaryotic cells with ribonuclease may be reversible in vivo.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: Cell treatment
• Molecular Mass: 49.8 kDa
• AA Sequence: MSLDIQSLDIQCEELSDARWAELLP LLQQCQVVRLDDCGLTEARCKDISS ALRVNPALAELNLRSNELGD VGVHCVLQGLQTPSCKIQKLSLQNC CLTGAGCGVLSSTLRTLPTLQELHL SDNLLGDAGLQLLCEGLLDP QCRLEKLQLEYCSLSAASCEPLASV LRAKPDFKELTVSNNDINEAGVRVL CQGLKDSPCQLEALKLESCG VTSDNCRDLCGIVASKASLRELALG SNKLGDVGMAELCPGLLHPSSRLRT LWIWECGITAKGCGDLCRVL RAKESLKELSLAGNELGDEGARLLC ETLLEPGCQLESLWVKSCSFTAACC SHFSSVLAQNRFLLELQISN NRLEDAGVRELCQGLGQPGSVLRVL WLADCDVSDSSCSSLAATLLANHSL RELDLSNNCLGDAGILQLVE
  SVRQPGCLLEQLVLYDIYWSEEMED RLQALEKDKPSLRVISTRTRPLEQK LISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: RNH1 ribonuclease/angiogenin inhibitor 1 [ Homo sapiens (human) ]
• Official Symbol: RNH1
• Synonyms: RAI; RNH
• Gene ID: 6050
• mRNA Refseq: NM_002939.4
• Protein Refseq: NP_002930.2
• MIM: 173320
• UniProt ID: P13489