Active Recombinant Full Length Human RPS6KB1 Protein, C-Flag-tagged

• Cat.No.: RPS6KB1-233HFL
• Product Overview: Recombinant Full Length Human RPS6KB1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: EMSA reaction
• Molecular Mass: 59 kDa
• AA Sequence: MRRRRRRDGFYPAPDFRDREAEDMA GVFDIDLDQPEDAGSEDELEEGGQL NESMDHGGVGPYELGMEHCE KFEISETSVNRGPEKIRPECFELLR VLGKGGYGKVFQVRKVTGANTGKIF AMKVLKKAMIVRNAKDTAHT KAERNILEEVKHPFIVDLIYAFQTG GKLYLILEYLSGGELFMQLEREGIF MEDTACFYLAEISMALGHLH QKGIIYRDLKPENIMLNHQGHVKLT DFGLCKESIHDGTVTHTFCGTIEYM APEILMRSGHNRAVDWWSLG ALMYDMLTGAPPFTGENRKKTIDKI LKCKLNLPPYLTQEARDLLKKLLKR NAASRLGAGPGDAGEVQAHP FFRHINWEELLARKVEPPFKPLLQS EEDVSQFDSKFTRQTPVDSPDDSTL SESANQVFLGFTYVAPSVLE SVKEKFSFEPKIRSPRRFIGSPRTP VSPVKFSPGDFWGRGASASTANPQT PVEYPMETSGIEQMDVTMSG
  EASAPLPIRQPNSGPYKKQAFPMIS KRPEHLRMNLTRTRPLEQKLISEED LAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Acute myeloid leukemia, ErbB signaling pathway, Fc gamma R-mediated phagocytosis, Insulin signaling pathway, mTOR signaling pathway, TGF-beta signaling pathway

Gene Information

• Gene Name: RPS6KB1 ribosomal protein S6 kinase B1 [ Homo sapiens (human) ]
• Official Symbol: RPS6KB1
• Synonyms: S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha
• Gene ID: 6198
• mRNA Refseq: NM_003161.4
• Protein Refseq: NP_003152.1
• MIM: 608938
• UniProt ID: P23443