Active Recombinant Full Length Human SAE1 Protein, C-Flag-tagged

• Cat.No.: SAE1-436HFL
• Product Overview: Recombinant Full Length Human SAE1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Posttranslational modification of proteins by the addition of the small protein SUMO, or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: ELISA capture for autoantibodies
• Molecular Mass: 38.3 kDa
• AA Sequence: MVEKEEAGGGISEEEAAQYDRQIRL WGLEAQKRLRASRVLLVGLKGLGAE IAKNLILAGVKGLTMLDHEQ VTPEDPGAQFLIRTGSVGRNRAEAS LERAQNLNPMVDVKVDTEDIEKKPE SFFTQFDAVCLTCCSRDVIV KVDQICHKNSIKFFTGDVFGYHGYT FANLGEHEFVEEKTKVAKVSQGVED GPDTKRAKLDSSETTMVKKK VVFCPVKEALEVDWSSEKAKAALKR TTSDYFLLQVLLKFRTDKGRDPSSD TYEEDSELLLQIRNDVLDSL GISPDLLPEDFVRYCFSEMAPVCAV VGGILAQEIVKALSQRDPPHNNFFF FDGMKGNGIVECLGPKTRTRPLEQK LISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: Ubiquitin mediated proteolysis

Gene Information

• Gene Name: SAE1 SUMO1 activating enzyme subunit 1 [ Homo sapiens (human) ]
• Official Symbol: SAE1
• Synonyms: AOS1; SUA1; UBLE1A; HSPC140
• Gene ID: 10055
• mRNA Refseq: NM_005500.3
• Protein Refseq: NP_005491.1
• MIM: 613294
• UniProt ID: Q9UBE0