Active Recombinant Full Length Human SOX9 Protein, C-Flag-tagged

• Cat.No.: SOX9-175HFL
• Product Overview: Recombinant Full Length Human SOX9 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: EMSA assay
• Molecular Mass: 56 kDa
• AA Sequence: MNLLDPFMKMTDEQEKGLSGAPSPT MSEDSAGSPCPSGSGSDTENTRPQE NTFPKGEPDLKKESEEDKFP VCIREAVSQVLKGYDWTLVPMPVRV NGSSKNKPHVKRPMNAFMVWAQAAR RKLADQYPHLHNAELSKTLG KLWRLLNESEKRPFVEEAERLRVQH KKDHPDYKYQPRRRKSVKNGQAEAE EATEQTHISPNAIFKALQAD SPHSSSGMSEVHSPGEHSGQSQGPP TPPTTPKTDVQPGKADLKREGRPLP EGGRQPPIDFRDVDIGELSS DVISNIETFDVNEFDQYLPPNGHPG VPATHGQVTYTGSYGISSTAATPAS AGHVWMSKQQAPPPPPQQPP QAPPAPQAPPQPQAAPPQQPAAPPQ QPQAHTLTTLSSEPGQSQRTHIKTE QLSPSHYSEQQQHSPQQIAY SPFNLPHYSPSYPPITRSQYDYTDH QNSSSYYSHAAGQGTGLYSTFTYMN PAQRPMYTPIADTSGVPSIP
  QTHSPQHWEQPVYTQLTRPTRTRPL EQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transcription Factors

Gene Information

• Gene Name: SOX9 SRY-box transcription factor 9 [ Homo sapiens (human) ]
• Official Symbol: SOX9
• Synonyms: CMD1; SRA1; CMPD1; SRXX2; SRXY10
• Gene ID: 6662
• mRNA Refseq: NM_000346.4
• Protein Refseq: NP_000337.1
• MIM: 608160
• UniProt ID: P48436