Active Recombinant Full Length Human SPOP Protein, C-Flag-tagged

• Cat.No.: SPOP-527HFL
• Product Overview: Recombinant Full Length Human SPOP Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a protein that may modulate the transcriptional repression activities of death-associated protein 6 (DAXX), which interacts with histone deacetylase, core histones, and other histone-associated proteins. In mouse, the encoded protein binds to the putative leucine zipper domain of macroH2A1.2, a variant H2A histone that is enriched on inactivated X chromosomes. The BTB/POZ domain of this protein has been shown in other proteins to mediate transcriptional repression and to interact with components of histone deacetylase co-repressor complexes. Alternative splicing of this gene results in multiple transcript variants encoding the same protein.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: MS digestion standard
• Molecular Mass: 42 kDa
• AA Sequence: MSRVPSPPPPAEMSSGPVAESWCYT QIKVVKFSYMWTINNFSFCREEMGE VIKSSTFSSGANDKLKWCLR VNPKGLDEESKDYLSLYLLLVSCPK SEVRAKFKFSILNAKGEETKAMESQ RAYRFVQGKDWGFKKFIRRD FLLDEANGLLPDDKLTLFCEVSVVQ DSVNISGQNTMNMVKVPECRLADEL GGLWENSRFTDCCLCVAGQE FQAHKAILAARSPVFSAMFEHEMEE SKKNRVEINDVEPEVFKEMMCFIYT GKAPNLDKMADDLLAAADKY ALERLKVMCEDALCSNLSVENAAEI LILADLHSADQLKTQAVDFINYHAS DVLETSGWKSMVVSHPHLVA
  EAYRSLASAQCPFLGPPRKRLKQST RTRPLEQKLISEEDLAANDILDYKD DDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: SPOP speckle type BTB/POZ protein [ Homo sapiens (human) ]
• Official Symbol: SPOP
• Synonyms: TEF2; BTBD32; NSDVS1; NSDVS2; NEDMACE; NEDMIDF
• Gene ID: 8405
• mRNA Refseq: NM_001007229.1
• Protein Refseq: NP_001007230.1
• MIM: 602650
• UniProt ID: O43791