Active Recombinant Full Length Human TNF Protein, C-Flag-tagged

• Cat.No.: TNF-13HFL
• Product Overview: Recombinant Full Length Human TNF Protein, fused to Flag-tag at C-terminus, was expressed in HEK293T.

Specification

• Description: This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, psoriasis, rheumatoid arthritis ankylosing spondylitis, tuberculosis, autosomal dominant polycystic kidney disease, and cancer. Mutations in this gene affect susceptibility to cerebral malaria, septic shock, and Alzheimer disease. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
• Source: HEK293T
• Species: Human
• Tag: Flag
• Form: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Bio-activity: Cell treatment
• Molecular Mass: 25.5 kDa
• AA Sequence: MSTESMIRDVELAEEALPKKTGGPQ GSRRCLFLSLFSFLIVAGATTLFCL LHFGVIGPQREEFPRDLSLI SPLAQAVRSSSRTPSDKPVAHVVAN PQAEGQLQWLNRRANALLANGVELR DNQLVVPSEGLYLIYSQVLF KGQGCPSTHVLLTHTISRIAVSYQT KVNLLSAIKSPCQRETPEGAEAKPW YEPIYLGGVFQLEKGDRLSA EINRPDYLDFAESGQVYFGIIAL TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Notes: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >0.05 µg/µL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Secreted Protein, Transcription Factors, Transmembrane
• Protein Pathways: Adipocytokine signaling pathway, Allograft rejection, Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Asthma, Cytokine-cytokine receptor interaction, Dilated cardiomyopathy, Fc epsilon RI signaling pathway, Graft-versus-host disease, Hematopoietic cell lineage, Hypertrophic cardiomyopathy (HCM), MAPK signaling pathway, Natural killer cell mediated cytotoxicity, NOD-like receptor signaling pathway, RIG-I-like receptor signaling pathway, Systemic lupus erythematosus, T cell receptor signaling pathway, TGF-beta signaling pathway, Toll-like receptor signaling pathway, Type I diabetes mellitus, Type II diabetes mellitus

Gene Information

• Gene Name: TNF tumor necrosis factor [ Homo sapiens (human) ]
• Official Symbol: TNF
• Synonyms: DIF; TNFA; TNFSF2; TNLG1F; TNF-alpha
• Gene ID: 7124
• mRNA Refseq: NM_000594.4
• Protein Refseq: NP_000585.2
• MIM: 191160
• UniProt ID: P01375