Active Recombinant Full Length Human ubiquitin like modifier activating enzyme 1 Protein, Flag Tagged

• Cat.No.: UBE1-05HFL
• Product Overview: Recombinant human full length UBE1 (Ubiquitin Activating Enzyme (E1), also known as UBA1, encompassing amino acids 2-1058 (end). This construct contains an N-terminal FLAG-tag. The recombinant protein was affinity purified.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: Flag
• Protein Length: 2-1058aa
• Description: The protein encoded by this gene catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternatively spliced transcript variants that encode the same protein have been described.
• Tag: N-Flag
• Form: Aqueous buffer solution.
• Bio-activity: 3 pmole/min/μg
• Molecular Mass: 119 kDa
• Purity: ≥90%
• Usage: Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.
• Applications: Useful in conjunction with E2 and E3 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Notes: Avoid freeze/thaw cycles
• Storage: At least months at -80 centigrade.
• Storage Buffer: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, 3 mM DTT, 80 μg/ml FLAG peptide.
• Shipping: At -80 centigrade.
• References: 1. Su Z.L. et al., Leuk. Lymphoma 49 (9), 1821-1822 (2008).
  2. Ramser J. et al., Am. J. Hum. Genet. 82 (1), 188-193 (2008).

Gene Information

• Gene Name: UBA1 ubiquitin like modifier activating enzyme 1 [ Homo sapiens (human) ]
• Official Symbol: UBA1
• Synonyms: UBA1; ubiquitin like modifier activating enzyme 1; A1S9; A1ST; GXP1; UBE1; A1S9T; AMCX1; POC20; SMAX2; UBA1A; UBE1X; VEXAS; CFAP124; ubiquitin-like modifier-activating enzyme 1; A1S9T and BN75 temperature sensitivity complementing; POC20 centriolar protein homolog; UBA1, ubiquitin-activating enzyme E1 homolog A; testicular secretory protein Li 63; EC 6.2.1.45
• Gene ID: 7317
• mRNA Refseq: NM_003334
• Protein Refseq: NP_003325
• MIM: 314370
• UniProt ID: P22314