Recombinant Human UBE1 protein, His-tagged

• Cat.No.: UBE1-0814H
• Product Overview: Recombinant Human UBE1 protein(Ser2-Arg1058), fused with His tag, was expressed in Baculovirus-Insect cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Protein Length: 2-1058 a.a.
• Form: Supplied as 0.2 μm filtered solution in 20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH7.5 with glycerol as protectant.
• Molecular Mass: This protein carries a polyhistidine tag at the N-terminus.The protein has a calculated MW of 119.8 kDa. The protein migrates as 80-110 kDa under reducing (R) condition (SDS-PAGE).
• Proteinlength: Ser2-Arg1058
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.