| Species : |
Thermotoga maritima |
| Source : |
E.coli |
| Tag : |
His |
| Description : |
α-L-Fucosidase, Microorganism (EC 3.2.1.51) is an enzyme that catalyzes the chemical reaction. Serum activity of α-L-Fucosidase, Microorganism, a lysosomal enzyme present in all mammalian cells, has been proposed as a marker of hepatocellular carcinoma (HCC). Thermophilic Fucosidase is active at a wide range of temperatures (37 to 97 centigrade), with an optimum of 70 centigrade or higher. The specificity of Thermophilic Fucosidase depends on linkage type and glycan substructure. |
| Reconstitution : |
Dissolve the lyophilized product in 100 μL of molecular grade water to make a 2,000 units/mL solution in 1× Reaction Buffer. Once reconstituted, store the enzyme at -20 to 4 centigrade for up to 1 month. Aliquoting is recommended to avoid repeated freeze-thaw cycles. |
| Activity assay : |
One unit of enzyme is added to 100 μL of 500 μM pNP-Fuc in 1× Reaction Buffer 7 (50 mM sodium citrate, pH 5.0) at 37 centigrade for 30 min, followed by addition of 100 μL of a stop solution (0.2 M sodium borate, pH 9.8). Measure absorption at 405 nm on a plate reader. |
| Unit Definition : |
One unit is defined as the amount of Thermophilic Fucosidase required to catalyze the release of 1 nmole p-nitrophenol (pNP) from p-nitrophenyl-α-L-fucopyranoside (pNP-Fuc) per min at 37 centigrade in 100 μL 1× Reaction Buffer (50 mM Tris, 100 mM NaCl, pH 7.5). |
