Active Recombinant H7N7 (A/Netherlands/219/03) HA Protein, His-tagged

Cat.No. : HA-5743V
Product Overview : Active Recombinant H7N7 (A/Netherlands/219/03) HA Protein(AAR02640.1)(Met 8-Phe 544) (Native, HA1+HA2) was expressed in Insect Cells, fused with a polyhistidine tag at the C-terminus.
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Species : H7N7
Source : Insect Cells
Tag : His
Protein Length : 8-544 aa
Form : Lyophilized from sterile PBS, pH 7.4.
Bio-activity : 1. Measured by its ability to agglutinate guinea pig red blood cells. HA titer is 0.05-0.3μg/ml for 1% GRBC. 2. Measured by its ability to bind with Neu5Aca2-3Galb1-4GlcNAcb-PAA-biotin (01-077) using the Octet RED System.
Molecular Mass : The secreted recombinant hemagglutinin of influenza A virus (A/Netherlands/219/03 (H7N7)) comprises 530 amino acids and has a predicted molecular mass of 59 kDa as estimated in SDS-PAGE under reducing conditions.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method.
Purity : > 87 % as determined by SDS-PAGE
Storage : Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Not For Human Consumption!

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