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Active Recombinant Human ACE2 Protein, FLAG-tagged, Biotinylated

Cat.No. : ACE2-10H
Product Overview : Biotinylated recombinant human Angiotensin-converting enzyme 2 (ACE2) expressed in HEK293 cells. Extracellular domain of human ACE2 (aa 18-740) are fused at the C-terminus to a FLAG®-tag.
  • Specification
  • Gene Information
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Description : The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. ACE2 is known to be expressed in various human organs, and its organ- and cell-specific expression suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronavirus HCoV-NL63 and the human severe acute respiratory syndrome coronaviruses, SARS-CoV and SARS-CoV-2, the latter is the causative agent of coronavirus disease-2019 (COVID-19). Multiple splice variants have been found for this gene and the dACE2 (or MIRb-ACE2) splice variant has been found to be interferon inducible.
Source : HEK293
Species : Human
Tag : FLAG
Bio-activity : ACE2 Human, HEK293 Recombinant binds with high affinity to the Spike S1RBD protein of the virus SARS-CoV-2.
ACE2 Human, HEK293 Recombinant was coated on an ELISA plate at 1μg/ml overnight at 4 centigrade. Recombinant SARS-CoV-2 Spike Protein S1RBD was added (starting at a concentration of 500 ng/mL with a two-fold serial dilution) during one hour at RT and the interaction was then detected using an anti-human IgG (HRP).
Molecular Mass : ~110kDa (SDS-PAGE)
~240kDa (Dimer, by SEC)
Protein length : 18-740
Endotoxin : <0.01 EU/μg (LAL test).
Purity : ≥ 95%
Applications : Cell culture and/or animal studies, In vitro, COVID-19
Storage : Stable for at least 6 months after receipt when stored at -20 centigrade. After opening, prepare aliquots and store at -20 centigrade. Working aliquots are stable for up to 3 months when stored at -20 centigrade. Avoid freeze/thaw cycles. For maximum product recovery after thawing, centrifuge the vial before opening the cap.
Storage Buffer : 1 mg/mL after reconstitution. Lyophilized. Contains PBS.
Reconstitution : Reconstitute with 50 μL endotoxin-free water.
Shipping : Shipped on ice packs.
Gene Name : ACE2 angiotensin converting enzyme 2 [ Homo sapiens (human) ]
Official Symbol : ACE2
Synonyms : ACE2; angiotensin converting enzyme 2; ACEH; angiotensin-converting enzyme 2; ACE-related carboxypeptidase; angiotensin I converting enzyme (peptidyl-dipeptidase A) 2; angiotensin I converting enzyme 2; angiotensin-converting enzyme homolog; angiotensin-converting enzyme-related carboxypeptidase; metalloprotease MPROT15; peptidyl-dipeptidase A; EC 3.4.17.23
Gene ID : 59272
mRNA Refseq : NM_021804
Protein Refseq : NP_068576
MIM : 300335
UniProt ID : Q9BYF1

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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