Active Recombinant Human ACE2 protein, mFc-tagged

Cat.No. : ACE2-185H
Product Overview : Recombinant Human ACE2 protein(Gln 18 - Ser 740), fused with mFc tag, was expressed in HEK293.
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Source : HEK293
Species : Human
Tag : mFc
Form : Supplied as 0.2 μm filtered solution in 50 mM Tris, 150 mM NaCl, Arginine, pH7.5 with glycerol as protectant.
Bio-activity : Immobilized SARS-CoV-2 S protein RBD, His Tag at 1 μg/mL (100 μL/well) can bind Human ACE2, Mouse IgG1 Fc Tag with a linear range of 0.4-6 ng/mL.Immobilized Human ACE2, Mouse IgG1 Fc Tag at 1 μg/mL (100 μL/well) can bind SARS-CoV-2 S1 protein, Fc Tag with a linear range of 1-20 ng/mL.
Molecular Mass : This protein carries a mouse IgG1 Fc tag at the C-terminusThe protein has a calculated MW of 109.8 kDa. The protein migrates as 125-150 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein Length : Gln 18 - Ser 740
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Publication :
Gene Name : ACE2 angiotensin I converting enzyme (peptidyl-dipeptidase A) 2 [ Homo sapiens ]
Official Symbol : ACE2
Synonyms : ACE2; angiotensin I converting enzyme (peptidyl-dipeptidase A) 2; angiotensin-converting enzyme 2; metalloprotease MPROT15; ACE-related carboxypeptidase; angiotensin I converting enzyme 2; angiotensin-converting enzyme homolog; ACEH;
Gene ID : 59272
mRNA Refseq : NM_021804
Protein Refseq : NP_068576
MIM : 300335
UniProt ID : Q9BYF1

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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