| Species : |
Human |
| Source : |
HEK293 |
| Tag : |
His |
| Protein Length : |
Biotin |
| Conjugation/Label : |
18-740 aa |
| Description : |
Angiotensin-converting enzyme 2 (ACE2) is a type I transmembrane zinc metalloprotease with about 60% homology to ACE, consisting of 805 amino acids including a signal peptide, catalytic domain, membrane anchor, and cytoplasmic tail. ACE2 regulates the renin-angiotensin system by converting angiotensin I to angiotensin 1-9 and angiotensin II to the vasodilator angiotensin 1-7, thus counteracting vasoconstriction and playing a key role in cardiovascular and renal function. ACE2 also serves as the functional receptor for human coronaviruses SARS-CoV, SARS-CoV-2, and HCoV-NL63, facilitating viral entry. Its expression is high in lung, heart, kidney, intestine, testis, and vascular cells. Beyond cardiovascular regulation, ACE2 is involved in respiratory disease pathogenesis, including acute respiratory distress syndrome, and has been linked to diabetes and hypertension. Additionally, its homolog collectrin is implicated in amino acid transport and genetic disorders like Hartnup disease. |
| Form : |
Liquid, 1×PBS buffer, pH7.4, 0.22 μm filtered |
| Molecular Mass : |
This protein has a predicted molecular weight of 86.5 kDa. Under DTT-reducing conditions, the protein migrates at approximately 90-100 kDa on SDS-PAGE. |
| Purity : |
>95% based on SDS-PAGE under reducing condition |
| Applications : |
ELISA, BLI |
| Storage : |
Briefly centrifuge the vial upon receipt. An unopened vial can be stored at 4 centigrade for up to 2 weeks, or at -20 centigrade or below for up to six months. The protein may be further diluted to 0.1 mg/mL using 0.22 μm-filtered PBS buffer (pH 7.4). For long-term storage, the diluted stock solution should be aliquoted and stored at ≤ –70 centigrade to minimize freeze-thaw cycles. If additional dilution is required, carrier proteins such as FBS or BSA should be added to maintain protein stability. |
| Concentration : |
25 μg size is bottled at 0.2 mg/mL concentration. 100 μg size is supplied at a lot-specific concentration. |
