Active Recombinant Human ACHE protein, His-tagged

• Cat.No.: ACHE-105H
• Product Overview: Recombinant Human ACHE protein(Glu 32 - Leu 614), fused with His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Glu 32 - Leu 614
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Measured by its ability to cleave Acetylthiocholine. The specific activity is >500 nmol/min/μg, as measured under the described conditions.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 66.5 kDa. The protein migrates as 65-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: ACHE acetylcholinesterase [ Homo sapiens ]
• Official Symbol: ACHE
• Synonyms: ACHE; acetylcholinesterase; acetylcholinesterase (YT blood group) , acetylcholinesterase (Yt blood group) , YT; Yt blood group; apoptosis-related acetylcholinesterase; YT; ACEE; ARACHE; N-ACHE
• Gene ID: 43
• mRNA Refseq: NM_000665
• Protein Refseq: NP_000656
• MIM: 100740
• UniProt ID: P22303