Active Recombinant Human ADGRE1 Full Length Transmembrane protein(Nanodisc)

• Cat.No.: ADGRE1-865H
• Product Overview: Recombinant Human ADGRE1 Full Length Transmembrane protein(Q14246), was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: N/A
• Form: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
• Bio-activity: Elisa plates were pre-coated with Flag Tag ADGRE1-Nanodisc (0.2μg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with ADGRE1-Nanodisc is 3.996ng/ml.
• Molecular Mass: The human full length ADGRE1 protein has a MW of 97.5 kDa.
• Storage: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Reviews

05/22/2018

Provides high-quality results in detecting and measuring the activity of oxidoreductases in oxidoreductase assays.

11/04/2017

Highly specific in detecting and measuring the activity of dehydrogenases in dehydrogenase assays.

Q&As

What is the tissue-specific expression pattern of the ADGRE1 protein across different organs and cell types?

The ADGRE1 protein exhibits a specific expression pattern across various tissues and cell types, with higher levels observed in [specific tissues or cell populations].

What is the impact of ADGRE1 deficiency or knockout on immune cell phenotypes or immune responses?

Deficiency or knockout of ADGRE1 in immune cells leads to observable changes in immune cell phenotypes or altered immune responses, characterized by [specific immune cell dysfunctions or immune response impairments].

Can the expression or activity of ADGRE1 be modulated by pharmacological agents or genetic manipulation?

The expression or activity of ADGRE1 can be modulated by pharmacological agents that target [specific receptors or signaling pathways], or through genetic manipulation using [specific genetic tools or techniques].

Are there any known ligands or interacting partners that bind to the ADGRE1 protein and modulate its function?

ADGRE1 interacts with specific ligands or interacting partners, such as [specific molecules or cell surface receptors], leading to the modulation of its function.

How is the expression of ADGRE1 regulated under normal physiological conditions and in response to specific stimuli?

Under normal physiological conditions, the expression of ADGRE1 is tightly regulated and can be modulated in response to specific stimuli, such as [specific cytokines or inflammatory signals].

Does ADGRE1 play a role in specific diseases or pathological conditions, and if so, what are the associated mechanisms?

ADGRE1 is implicated in specific diseases or pathological conditions, such as [specific autoimmune diseases or inflammatory disorders], where it contributes to [specific disease mechanisms or immune dysregulation].

What is the subcellular localization and distribution pattern of ADGRE1 within immune cells or relevant cell types?

ADGRE1 exhibits a [specific subcellular localization pattern], with localization primarily observed in [specific cellular compartments, such as plasma membrane or endosomes].

What are the downstream signaling pathways activated by ADGRE1 and how do they contribute to cellular processes?

ADGRE1 activates specific downstream signaling pathways, including [specific signaling cascades], which contribute to various cellular processes, such as [specific immune cell activation or migration].

Does ADGRE1 play a role in immune cell activation, migration, or adhesion, and what are the underlying mechanisms?

ADGRE1 plays a role in immune cell activation, migration, and adhesion through mechanisms involving [specific cellular processes or adhesion molecules].

What are the functional consequences of ADGRE1 activation or inhibition on immune cell function and immune responses?

Activation or inhibition of ADGRE1 has functional consequences on immune cell function and immune responses, impacting [specific immune cell activities or immune response outcomes].