AKR1B10-995H
Optional Service: Optional requirements on this protein
Product Overview :
Recombinant Human AKR1B10 (Accession # O60218) Ala2-Tyr316, fused with an N-terminal Met and 6-His tag, was produced in Spodoptera frugiperda, Sf 21 (baculovirus)-derived.
Source :
Baculovirus Sf21 Insect Cells
Species :
Human
Tag :
& His
Predicted N Terminal :
Inconclusive result, Met predicted. Protein identity confirmed by MS analysis of tryptic fragments
Form :
Supplied as a 0.2 µm filtered solution in Tris, NaCl, DTT and Glycerol.
Bio-activity :
Measured by the disappearance of NADPH during the reducation of 4-nitrobenzaldehyde.The specific activity is >1,000 pmol/min/µg, as measured under the described conditions.
Molecular Mass :
Recombinant Human AKR1B10, Met & His-tagged has a calculated MW of 37 kDa. In SDS-PAGE migrates as 37-38 kDa, reducing conditions.
Protein length :
Ala2-Tyr316
Purity :
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
Storage :
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitution :
Reconstitute at 100 µg/mL in PBS containing at least 0.1% human or bovine serum albumin.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.