Active Recombinant Human AKR1B10 Protein, Met & His-tagged

• Cat.No.: AKR1B10-995H
• Product Overview: Recombinant Human AKR1B10 (Accession # O60218) Ala2-Tyr316, fused with an N-terminal Met and 6-His tag, was produced in Spodoptera frugiperda, Sf 21 (baculovirus)-derived.

Specification

• Species: Human
• Source: Sf21 Cells
• Tag: His
• Protein Length: Ala2-Tyr316
• Predicted N Terminal: Inconclusive result, Met predicted. Protein identity confirmed by MS analysis of tryptic fragments
• Form: Supplied as a 0.2 µm filtered solution in Tris, NaCl, DTT and Glycerol.
• Bio-activity: Measured by the disappearance of NADPH during the reducation of 4-nitrobenzaldehyde.The specific activity is >1,000 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Human AKR1B10, Met & His-tagged has a calculated MW of 37 kDa. In SDS-PAGE migrates as 37-38 kDa, reducing conditions.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: AKR1B10 aldo-keto reductase family 1, member B10 (aldose reductase) [ Homo sapiens ]
• Official Symbol: AKR1B10
• Synonyms: AKR1B10; aldo-keto reductase family 1, member B10 (aldose reductase); AKR1B11; aldo-keto reductase family 1 member B10; AKR1B12; aldo keto reductase family 1; member B11 (aldose reductase like); aldose reductase like 1; aldose reductase like peptide; aldose reductase related protein; ALDRLn; ARL 1; ARL1; HIS; HSI; small intestine reductase; ARP; hARP; SI reductase; aldose reductase-like 1; aldose reductase-like peptide; aldose reductase-related protein; aldo-keto reductase family 1, member B11 (aldose reductase-like); ARL-1; MGC14103
• Gene ID: 57016
• mRNA Refseq: NM_020299
• Protein Refseq: NP_064695
• MIM: 604707
• UniProt ID: O60218
• Chromosome Location: 7q33