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Recombinant Human AKR1B10 293 Cell Lysate

Cat.No. : AKR1B10-8931HCL
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  • Gene Information
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Description : Antigen standard for aldo-keto reductase family 1, member B10 (aldose reductase) (AKR1B10) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : AKR1B10 aldo-keto reductase family 1, member B10 (aldose reductase) [ Homo sapiens ]
Official Symbol : AKR1B10
Synonyms : AKR1B10; aldo-keto reductase family 1, member B10 (aldose reductase); AKR1B11; aldo-keto reductase family 1 member B10; AKR1B12; aldo keto reductase family 1; member B11 (aldose reductase like); aldose reductase like 1; aldose reductase like peptide; aldose reductase related protein; ALDRLn; ARL 1; ARL1; HIS; HSI; small intestine reductase; ARP; hARP; SI reductase; aldose reductase-like 1; aldose reductase-like peptide; aldose reductase-related protein; aldo-keto reductase family 1, member B11 (aldose reductase-like); ARL-1; MGC14103;
Gene ID : 57016
mRNA Refseq : NM_020299
Protein Refseq : NP_064695
MIM : 604707
UniProt ID : O60218
Chromosome Location : 7q33
Pathway : Fructose and mannose metabolism, organism-specific biosystem; Fructose and mannose metabolism, conserved biosystem; Galactose metabolism, organism-specific biosystem; Galactose metabolism, conserved biosystem; Glycerolipid metabolism, organism-specific biosystem; Glycerolipid metabolism, conserved biosystem; Metabolic pathways, organism-specific biosystem;
Function : aldo-keto reductase (NADP) activity; geranylgeranyl reductase activity; indanol dehydrogenase activity; protein binding; retinal dehydrogenase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (15)

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What are the methods for laboratory detection of AKR1B10 protein? 03/25/2023

AKR1B10 protein is mainly detected in laboratory by enzyme-linked immunosorbent assay, fluorescence quantitative PCR and other methods.

Which cell signaling pathways does AKR1B10 protein participate in? 09/10/2022

AKR1B10 is involved in a variety of cell signaling pathways, such as NF-κB, WNT/ β-catenin signaling pathways, and thus plays a role in cell proliferation, differentiation and apoptosis.

How does AKR1B10 play a role in neuroprotective mechanisms? 06/03/2022

This protein is involved in the NADP/NADPH metabolic pathway, plays an antioxidant and neuroprotective role, and thus has a promising application in the prevention and treatment of central nervous system diseases.

AKR1B10 has the potential to be used in the early diagnosis and prevention of which cancers? 02/06/2022

AKR1B10 has a certain application prospect in the early diagnosis and prognosis evaluation of lung cancer, colorectal cancer, liver cancer and other cancers.

How is AKR1B10 protein distributed in the human body 01/24/2022

The AKR1B10 protein is an alcohol contraction enzyme that is associated with a variety of diseases and is mainly found in tissues such as liver, lung, stomach, breast, kidney, pancreas and intestine.

Does AKR1B10 have potential applications in the treatment of inflammatory diseases? 08/31/2021

AKR1B10 inhibitors can be used to prevent and treat a variety of inflammatory diseases, such as rheumatoid arthritis and hepatitis B.

How does AKR1B10 function in lung cancer? 08/28/2021

AKR1B10 protein is associated with cell proliferation, angiogenesis and malignant metastasis in lung cancer

In what diseases do AKR1B10 inhibitors have potential applications? 06/18/2021

AKR1B10 inhibitors have potential anti-tumor and anti-inflammatory therapeutic effects and can be used in the treatment of lung cancer, breast cancer, diabetes and other diseases.

Whether AKR1B10 protein is involved in tumorigenesis? 05/03/2021

The expression level of AKR1B10 protein in tumor cells is increased, which is related to tumor proliferation, invasion and metastasis, and is a new tumor biomarker.

What is the role of AKR1B10 protein in metabolic diseases? 04/01/2021

AKR1B10 is involved in the occurrence and development of metabolic diseases, such as diabetes and obesity, and is a possible therapeutic target.

Whether AKR1B10 protein has a function in the human body 07/18/2020

AKR1B10 protein is involved in various metabolic processes and cell proliferation and growth, such as steroid hormone metabolism, fatty acid synthesis, cell cycle regulation and so on.

Does there any steroid hormone metabolic pathways involved in AKR1B10? 08/18/2019

The metabolic pathways of steroid hormones involved in AKR1B10 include oxidative metabolism and reductive metabolism of steroid hormones.

Is AKR1B10 protein associated with hepatotoxicity? 07/16/2019

AKR1B10 protein is involved in the metabolism of some drugs and may be related to hepatotoxicity, which is a hot molecule in the study of drug metabolism and toxicity.

What diseases is AKR1B10 associated with? 04/24/2019

AKR1B10 protein is associated with a variety of diseases, such as tumors, lung diseases, metabolic diseases, and neurological diseases.

In what retinal diseases is AKR1B10 associated? 04/14/2019

AKR1B10 protein is involved in the metabolism of retinal glycoglycation end products, which is associated with diabetic retinopathy and other diseases.

Customer Reviews (4)

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Reviews
09/20/2022

    Has therapeutic effects on specific disease models and is a promising candidate for new drugs.

    03/31/2022

      The laboratory personnel feedback that the protein is easy to operate, can meet the experimental needs, and has a good cost performance.

      05/30/2019

        It has been found to increase yield and purity and improve production efficiency in biotechnology production.

        09/30/2018

          Good fluorescence labeling performance in light microscopy technology, which is helpful for cell imaging studies.

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