Active Recombinant Human AKR1C4 protein, His-tagged

• Cat.No.: AKR1C4-1006H
• Product Overview: Recombinant Human AKR1C4(1-323aa) fused with His tag at N-terminal was expressed in E. coli, bioactivity Validated.

Specification

• Description: AKR1C4 belongs to aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. It catalyzes the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. This enzyme catalyzes the bioreduction of chlordecone, a toxic organochlorine pesticide, to chlordecone alcohol in liver.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT
• Bio-activity: Specific activity is > 700 pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction of 1.0 pmole 3-chlorobenzaldehyde presence of NADP per minute at pH 8.8 at 25 centigrade.
• Molecular Mass: 39.2 kDa(343aa), confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MDPKYQRVEL NDGHFMPVLG FGTYAPPEVP RNRAVEVTKL AIEAGFRHID SAYLYNNEEQ VGLAIRSKIADGSVKREDIF YTSKLWCTFF QPQMVQPALE SSLKKLQLDY VDLYLLHFPM ALKPGETPLP KDENGKVIFD TVDLSATWEV MEKCKDAGLAKSIGVSNFNC RQLEMILNKP GLKYKPVCNQ VECHPYLNQS KLLDFCKSKD IVLVAHSALG TQRHKLWVDP NSPVLLEDPV LCALAKKHKRTPALIALRYQ LQRGVVVLAK SYNEQRIREN IQVFEFQLTS EDMKVLDGLN RNYRYVVMDF LMDHPDYPFS DEY
• Purity: > 90% by SDS - PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1.0 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: AKR1C4 aldo-keto reductase family 1, member C4 (chlordecone reductase; 3-alpha hydroxysteroid dehydrogenase, type I; dihydrodiol dehydrogenase 4) [ Homo sapiens ]
• Official Symbol: AKR1C4
• Synonyms: AKR1C4; aldo-keto reductase family 1, member C4 (chlordecone reductase; 3-alpha hydroxysteroid dehydrogenase, type I; dihydrodiol dehydrogenase 4); CHDR; aldo-keto reductase family 1 member C4; 3 alpha HSD; C11; CDR; DD4; HAKRA; MGC22581; 3-alpha-HSD1; dihydrodiol dehydrogenase isozyme DD4; type I 3-alpha-hydroxysteroid dehydrogenase; DD-4; 3-alpha-HSD
• Gene ID: 1109
• mRNA Refseq: NM_001818
• Protein Refseq: NP_001809
• MIM: 600451
• UniProt ID: P17516
• Chromosome Location: 10p15.1
• Pathway: Bile acid and bile salt metabolism, organism-specific biosystem; Bile acid biosynthesis, cholesterol => cholate, organism-specific biosystem; Bile acid biosynthesis, cholesterol => cholate, conserved biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem
• Function: aldo-keto reductase (NADP) activity; androsterone dehydrogenase (B-specific) activity; androsterone dehydrogenase activity; bile acid transmembrane transporter activity; chlordecone reductase activity; electron carrier activity; oxidoreductase activity; oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor; retinal dehydrogenase activity