Active Recombinant Human ATP1B1 Protein (63-303aa), C-His tagged

• Cat.No.: ATP1B1-17H
• Product Overview: Recombinant human ATP1B1 protein (63-303aa), fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specification

• Description: The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 1 subunit. Alternatively spliced transcript variants encoding different isoforms have been described, but their biological validity is not known.
• Source: Baculovirus
• Species: Human
• Tag: C-His
• Form: Liquid
• Bio-activity: > 3,000 pmol/min/μg, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of Adenosine 5-triphosphate to phosphate per minute per minute at pH 7.5 at 25 centigrade.
• Molecular Mass: 29 kDa (250aa)
• Protein Length: 63-303aa
• AA Sequence: EFKPTYQDRVAPPGLTQIPQIQKTE ISFRPNDPKSYEAYVLNIVRFLEKY KDSAQRDDMIFEDCGDVPSEPKERG DFNHERGERKVCRFKLEWLGNCSGL NDETYGYKEGKPCIIIKLNRVLGFK PKPPKNESLETYPVMKYNPNVLPVQ CTGKRDEDKDKVGNVEYFGLGNSPG FPLQYYPYYGKLLQPKYLQPLLAVQ FTNLTMDTEIRIECKAYGENIGYSE KDRFQGRFDVKIEVKS
• Endotoxin: < 1 EU/μg of protein (determined by LAL method)
• Purity: > 90% by SDS-PAGE
• Applications: SDS-PAGE, Enzyme Activity
• Notes: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/mL (determined by absorbance at 280nm)
• Storage Buffer: Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Gene Information

• Gene Name: ATP1B1 ATPase Na+/K+ transporting subunit beta 1 [ Homo sapiens (human) ]
• Official Symbol: ATP1B1
• Synonyms: ATP1B1; ATPase Na+/K+ transporting subunit beta 1; ATP1B; sodium/potassium-transporting ATPase subunit beta-1; ATPase, Na+/K+ transporting, beta 1 polypeptide; Beta 1-subunit of Na(+),K(+)-ATPase; Na, K-ATPase beta-1 polypeptide; adenosinetriphosphatase; sodium pump subunit beta-1; sodium-potassium ATPase subunit beta 1 (non-catalytic); sodium/potassium-dependent ATPase beta-1 subunit; sodium/potassium-transporting ATPase beta-1 chain; EC 3.6.1.3
• Gene ID: 481
• mRNA Refseq: NM_001677
• Protein Refseq: NP_001668
• MIM: 182330
• UniProt ID: P05026