Active Recombinant Human BMP2 Protein (Gln283-Arg396), GMP Grade

• Cat.No.: BMP2-08HG
• Product Overview: GMP recombinant human BMP-2 protein (Gln283-Arg396) without tag was expressed in Chinese Hamster Ovary cell line and manufactured and tested under current Good Manufacturing Practice (GMP) guidelines.

Specification

• Description: This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer, which plays a role in bone and cartilage development. Duplication of a regulatory region downstream of this gene causes a form of brachydactyly characterized by a malformed index finger and second toe in human patients.
• Source: CHO
• Species: Human
• Form: Disulfide-linked homodimer
• Bio-activity: Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 40-200 ng/mL.
  The specific activity of recombinant human BMP-2 is >5.0 x 105 units/mg, which is calibrated against the human BMP-2 WHO Standard.
• Molecular Mass: Predicted Molecular Mass: 13 kDa (monomer)
  SDS-PAGE: 15-16, kDa, reducing conditions
• Protein Length: Gln283-Arg396
• N-terminal Sequence Analysis: Gln-Ala-Lys-His-Lys-Gln-Arg-Lys-Arg-Leu
• Endotoxin: <0.10 EU/ μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE with silver staining, under reducing conditions.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  A minimum of 12 months when stored at ≤ -20 centigrade as supplied. Refer to lot specific COA for the Use by Date.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, ≤ -20 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in Glycine, Sucrose, Tween® 80 and Glutamic Acid.
• Reconstitution: Reconstitute at 100 μg/mL in 4 mM HCl.
• Shipping: The product is shipped with polar packs.

Gene Information

• Gene Name: BMP2 bone morphogenetic protein 2 [ Homo sapiens ]
• Official Symbol: BMP2
• Synonyms: BMP2; bone morphogenetic protein 2; BMP2A; BMP-2A; bone morphogenetic protein 2A; BDA2
• Gene ID: 650
• mRNA Refseq: NM_001200
• Protein Refseq: NP_001191
• MIM: 112261
• UniProt ID: P12643