Active Recombinant Human Bone Morphogenetic Protein 1, His-tagged

• Cat.No.: BMP1-571H
• Product Overview: Recombinant Human BMP1protein was expressed in Murine myeloma cell line with a C-terminal 6-His tag.Ala121-Gln730.

Specification

• Cat. No.: BMP1-571H
• Description: Bone morphogeneticprotein 1 (BMP1), also known as procollagen Cproteinase (PCP), is a zinc proteaseof the astacin family. BMP1/PCP plays a key role in formation ofextracellular matrix (ECM) by converting precursor proteins into their matureand functional forms. The precursor proteins identified as substrates forBMP1/PCP include collagens, biglycan, laminin 5, dentin matrix protein1, andlysyl oxidase. There are six alternatively spliced forms known to be derivedfrom the BMP1 gene, and isoform 1 consisting of residues 1 to 730 wasexpressed.
• Source: Mammalian cells
• Form: Lyophilized from a0.2 μm filtered solution in HEPES, NaCl, CaCl2, ZnCl2and Brij-35.
• N-terminalSequence: Ala121
• Molecular Weight: 70.5 kDa
• Activity: Measured by itsability to cleave a fluorogenic peptide substrate, Mca-YVADAPK (Dnp)-OH. Thespecific activity is > 4 pmol/min/μg, as measured under the describedconditions.
• Endotoxin Level: < 1.0EU per 1 μg of the protein by the LAL method.
• SDS-PAGE: 84 kDa, reducingconditions.
• Purity: > 95%, by SDSPAGEunder reducing conditions and visualized by silver stain.
• Reconstitution: 100 μg/mL in sterile25 mM HEPES and 0.01% Brij-35, pH 7.5.
• Storage: Use a manual defrostfreezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt,-20 to -70°C as supplied. 3 months, -20 to -70°C under sterile conditionsafter reconstitution.
• Protein length: Ala121-Gln730

Gene Information

• Gene Name: BMP1 bone morphogenetic protein 1[ Homo sapiens ]
• Official Symbol: BMP1
• Synonyms: BMP1; bonemorphogenetic protein 1; procollagen C-proteinase; mammalian tolloid protein;procollagen C-endopeptidase; BMP-1; PCP; PCOLC; mTId; EC 3.4.24.19
• Gene ID: 649
• mRNA Refseq: NM_001199
• Protein Refseq: NP_001190
• MIM: 112264
• UniProt ID: P13497
• Chromosome Location: 8p21
• Pathway: Adipogenesis;HDL-mediated lipid transport; Lipid digestion; Lipoprotein metabolism;Metabolism; Metabolism of lipids and lipoproteins
• Function: calcium ion binding;cytokine activity; growth factor activity; metal ion binding;metalloendopeptidase activity; metallopeptidase activity; peptidase activity;zinc ion binding