Active Recombinant Human C1QB Protein, His-SUMO-tagged

• Cat.No.: C1QB-598H
• Product Overview: Active Recombinant Human C1QB Protein(28-253 aa), fused with N-terminal His and SUMO tag, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His&SUMO
• Protein Length: 28-253 aa
• Form: PBS, pH7.4, containing 0.01% SKL, 5%Trehalose.
• Bio-activity: C1qB protein is a component of the C1 complex in the classical pathway of the complement system. It is a subunit of the C1q molecule, which is the first component of the complement system to initiate the classical pathway. C1q is a hexameric protein composed of six identical subunits, each containing a C1qB, a C1qB, and a C1qC subunit. C1qB plays a key role in eliminating pathogens, regulating inflammatory response and maintaining immune tolerance. The binding of C4b and C1qB is a key step in the activation of the classical pathway in the complement system, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human C1qB and recombinant mouse C4b. Briefly, C1qB was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μ l were then transferred to C4b-coated microtiter wells and incubated for 1h at 37°C. Wells were washed with PBST and incubated for 1h with anti-C1qB pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37°C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C . Finally, add 50 µL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human C1qB and recombinant mouse C4b was shown, the EC50 for this effect is 0.12 ug/mL.
• Molecular Mass: 37.5kDa
• Endotoxin: <1.0EU per 1μg (determined by the LAL method).
• Purity: >90%
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 month.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: C1QB complement component 1, q subcomponent, B chain [ Homo sapiens ]
• Official Symbol: C1QB
• Synonyms: C1QB; complement component 1, q subcomponent, B chain; complement component 1, q subcomponent, beta polypeptide; complement C1q subcomponent subunit B; complement component C1q, B chain; complement subcomponent C1q chain B
• Gene ID: 713
• mRNA Refseq: NM_000491
• Protein Refseq: NP_000482
• MIM: 120570
• UniProt ID: P02746