Active Recombinant Human CASP1 Protein

• Cat.No.: CASP1-596H
• Product Overview: Produced in E. coli. cDNA encodes residues identical to Asn120-His404 (C-terminus), except for an Asp381 to Glu change, introduced to stabilize the enzyme against autoproteolysis.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Protein Length: 120-404 aa
• Description: This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing results in transcript variants encoding distinct isoforms.
• Form: Liquid
• Molecular Mass: ~20 + 10 kDa
• Bio-activity: One U=1 pmol/min, using Ac-YVAD-pNA (200μM) as substrate, at 30 centigrade.
• Optimal pH: 0
• Isoelectric Point: 0
• Purity: ≥ 90% by SDS-PAGE
• Application: Useful tool to study enzyme regulation and kinetics, cleave target substrates, screen for inhibitors.
• Stability: After initial defrost, aliquot product into individual tubes and refreeze at -80 centigrade. Avoid repeated freeze/defrost cycles.The enzyme is stable on ice for the time typically required to set up an experiment (30-60 min.), but may lose activity with prolonged storage on ice. It is recommended that thawing and dilution of the enzyme be done within as short a time as possible before start of the assay. The remaining, undiluted and unused enzyme should be refrozen quickly by, for example, snap-freezing in a dry ice ethanol bath or liquid nitrogen. The enzyme is stable to at least 4 freeze/thaw cycles.
• Storage: Long term storage at -80 centigrade
• Storage Buffer: In 50mM HEPES, pH 7.4, containing 100mM sodium chloride, 0.5% CHAPS, 1mM EDTA, 10% glycerol and 10mM DTT.
• Shipping: Dry Ice

Gene Information

• Gene Name: CASP1 caspase 1 [ Homo sapiens (human) ]
• Official Symbol: CASP1
• Synonyms: CASP1; caspase 1, apoptosis-related cysteine peptidase; caspase 1, apoptosis related cysteine peptidase (interleukin 1, beta, convertase) , caspase 1, apoptosis related cysteine protease (interleukin 1, beta, convertase) , IL1BC; caspase-1; caspase 1; ICE; interleukin 1; beta; convertase; IL1B-convertase; CASP1 nirs variant 1; IL-1 beta-converting enzyme; interleukin 1, beta, convertase; interleukin 1-B converting enzyme; caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase); P45; IL1BC
• Gene ID: 834
• mRNA Refseq: NM_001223
• Protein Refseq: NP_001214
• MIM: 147678
• UniProt ID: P29466