Active Recombinant Human Caspase-5 Enzyme

• Cat.No.: CASP5-32H

Specification

• Species: Human
• Form: Lyophilized powder
• Bio-activity: 5000 units/mg
• Purity: ≥95% by SDS-PAGE
• Unit Definition: One unit of the recombinant caspase-5 is the enzyme activity that cleaves 1 nmol of the caspase substrate WEHD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM HEPES, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% glycerol and 10 mM DTT.
• Applications: Active caspase-5 is useful in studying enzyme regulation, determining target substrates, screening caspase inhibitors, or as a positive control in caspase activity assays. We recommend using 1 unit/assay for analyzing caspase activity.
• Usage: For in vitro research use only. Not for use in diagnostics or in humans.
• Storage: The lyophilized caspase-5 is stable for 1 year at -70°C. Avoid multiple freeze/thaw cycles as activity might decrease. Following reconstitution in PBS, the enzyme should be aliquoted and immediately stored at -70°C.
• Reconstitution: Reconstitute to 1 unit per μL in PBS containing 15% glycerol.
• Warning: No warranties, expressed or implied, are made regarding the use of this product. Creative Biomart is not liable for any damage, personal injury, or economic loss caused by this product.

Gene Information

• Gene Name: CASP5 caspase 5, apoptosis-related cysteine peptidase [ Homo sapiens ]
• Official Symbol: CASP5
• Synonyms: CASP5; caspase 5, apoptosis-related cysteine peptidase; caspase 5, apoptosis related cysteine protease; caspase-5; ICE(rel)III; CASP-5; TY protease; protease TY; ICE(rel)-III; protease ICH-3; caspase 5, apoptosis-related cysteine protease; ICH-3; ICEREL-III; MGC141966
• Gene ID: 838
• mRNA Refseq: NM_001136109
• Protein Refseq: NP_001129581
• MIM: 602665
• UniProt ID: P51878
• Chromosome Location: 11q22.2-q22.3
• Pathway: NOD-like receptor signaling pathway, organism-specific biosystem; NOD-like receptor signaling pathway, conserved biosystem
• Function: cysteine-type endopeptidase activity; cysteine-type peptidase activity; peptidase activity