Active Recombinant Human CCL2 protein

• Cat.No.: CCL2-0621H
• Product Overview: Recombinant Human CCL2 protein(Gln24-Thr99) was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Protein Length: 24-99 aa
• Form: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
• Bio-activity: 1. Measured by the ability to inhibit the proliferation of HUVEC(Human Umbilical Vein Endothelial Cells).The ED50 for this effect is 0.53-2.12 ng/mL. 2. Measured by its ability to chemoattract THP-1 cells. The ED50 for this effect is typically 1.01-4.04 ng/mL.
• Molecular Mass: 8.69 kDa
• Endotoxin: < 1 EU/μg of the protein by LAL method.
• Purity: ≥ 90 % as determined by SDS-PAGE.
• Storage: Store at -20°C.Store the lyophilized protein at -20°C to -80°C up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
• Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Gene Information

• Gene Name: CCL2 chemokine (C-C motif) ligand 2 [ Homo sapiens ]
• Official Symbol: CCL2
• Synonyms: CCL2; chemokine (C-C motif) ligand 2; SCYA2, small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig je); C-C motif chemokine 2; GDCF 2; HC11; MCAF; MCP 1; MCP1; MGC9434; monocyte chemoattractant protein 1; monocyte chemotactic and activating factor; monocyte chemotactic protein 1; homologous to mouse Sig je; monocyte secretory protein JE; small inducible cytokine subfamily A (Cys Cys); member 2; SMC CF; small-inducible cytokine A2; monocyte chemoattractant protein-1; small inducible cytokine subfamily A (Cys-Cys), member 2; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je); MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30
• Gene ID: 6347
• mRNA Refseq: NM_002982
• Protein Refseq: NP_002973
• UniProt ID: P13500