Active Recombinant Rat Chemokine (C-C motif) Ligand 2

• Cat.No.: Ccl2-469R
• Product Overview: Recombinant Rat Ccl2protein was expressed in Murine myeloma cell line. Gln24-Asn148.

Specification

• Cat. No.: Ccl2-469R
• Description: Rat Ccl2 is a memberof the β (CC) subfamily of chemokines that is a chemoattractant for monocytesand basophils but not eosinophils or neutrophils. Rat Ccl2 is secreted as a 14kDa glycoprotein monomer but noncovalent dimers probably occur. The first fiveamino acids of the mature protein are essential for activity; deletion of theN-terminal glutamine, which is pyrrolidone carboxylic acidmodified, dramaticallydecreases activity on basophils and, surprisingly, stimulates eosinophil chemotaxis.
• Source: Mammalian cells
• Form: Lyophilized from a 0.2μm filtered solution in PBS with Trehalose and with BSA as a carrier protein.
• N-terminalSequence: No results obtained:Gln24 predicted
• Molecular Weight: 14 kDa
• Activity: Measuredby its ability to chemoattract human CCR2A transfected BaF3 mouse proB cells.The ED50 for this effect is typically 30-150 ng/mL.
• Endotoxin Level: <1.0 EUper 1 μg of the protein by the LAL method.
• Purity: >90%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: Reconstituteat 10 μg/mL in sterile PBS that containing at least 0.1% human or bovine serumalbumin.
• Storage: Use a manualdefrost freezer and avoid repeated freezethaw cycles. 12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months,-20 to -70°C under sterile conditions after reconstitution.

Gene Information

• Gene Name: Ccl2 chemokine(C-C motif) ligand 2 [ Rattus norvegicus ]
• Official Symbol: Ccl2
• Synonyms: Ccl2; chemokine(C-C motif) ligand 2; MCP-1; Scya2; Sigje; C-C motif chemokine 2; small induciblegene JE; small inducible cytokine A2; small-inducible cytokine A2; monocyte chemotacticprotein 1; monocyte chemoattractant protein 1; monocyte chemoattractant protein-1;immediate-early serum-responsive JE protein; immediate-early serum-responsiveprotein JE; Je; Mcp1
• Gene ID: 24770
• mRNA Refseq: NM_031530
• Protein Refseq: NP_113718
• UniProt ID: P14844
• Chromosome Location: 10q26
• Pathway: Chemokine signaling pat
• Function: CCR2 chemokine receptorbinding; G-protein coupled receptor binding; chemokine activity; heparin binding