Active Recombinant Human CCL22 Protein (69 aa)

• Cat.No.: CCL22-081C
• Product Overview: Recombinant Human CCL22 Protein without tag was expressed in E. coli.

Specification

• Description: MDC is a CC chemokine that is produced in B cells, macrophages, monocyte-derived dendritic cells, activated NK cells and CD4 T cells. It signals through the CCR4 receptor. MDC chemoattracts monocytes, dendritic cells and NK cells and exerts HIV suppressive activity.
• Source: E. coli
• Species: Human
• Form: Sterile Filtered White lyophilized (freeze-dried) powder.
• Bio-activity: Fully biologically active when compared to standard. Determined by its ability to chemoattract human T cells using a concentration range of 10.0-100.0 ng/mL, corresponding to a Specific Activity of ＞1 × 10^4 IU/mg.
• Molecular Mass: 8.1 kDa, a single, non-glycosylated polypeptide chain containing 69 amino acids.
• Protein length: 69
• AA Sequence: GPYGANMEDSVCCRDYVRYRLPLRV VKHFYWTSDSCPRPGVVLLTFRDKE ICADPRVPWVKMILNKLSQ
• Endotoxin: Less than 1 EU/mg of rHuMDC/CCL22 as determined by LAL method.
• Purity: >97% by SDS-PAGE and HPLC analyses.
• Storage: This lyophilized preparation is stable for several weeks at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 to -70 centigrade. Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2mm filtered concentrated solution in 20mM PB, pH7.4, 500mM NaCl.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at < -20 centigrade. Further dilutions should be made in appropriate buffered solutions.

Gene Information

• Gene Name: CCL22 chemokine (C-C motif) ligand 22 [ Homo sapiens ]
• Official Symbol: CCL22
• Synonyms: CCL22; chemokine (C-C motif) ligand 22; SCYA22, small inducible cytokine subfamily A (Cys Cys), member 22; C-C motif chemokine 22; A 152E5.1; ABCD 1; DC/B CK; MDC; MGC34554; STCP 1; MDC(1-69); CC chemokine STCP-1; macrophage-derived chemokine; small inducible cytokine A22; small-inducible cytokine A22; stimulated T cell chemotactic protein 1; stimulated T-cell chemotactic protein 1; small inducible cytokine subfamily A (Cys-Cys), member 22; ABCD-1; SCYA22; STCP-1; DC/B-CK; A-152E5.1
• Gene ID: 6367
• mRNA Refseq: NM_002990
• Protein Refseq: NP_002981
• MIM: 602957
• UniProt ID: O00626

Reviews

05/01/2022

The catalytic activity of CCL22 is very strong, which can significantly improve the selectivity and yield of chemical reactions, and has high application value.

03/13/2022

This repeatability of CCL22 is very good, which can ensure the safety of the experiment and avoid unnecessary risks.

08/25/2019

CCL22 were well suited for our research needs due to their simple storage conditions and long-term preservation.

Q&As

What are the gene expression regulatory mechanisms of CCL22 protein?

The expression of CCL22 gene is regulated by a variety of signaling pathways, including inflammatory factor-mediated signaling pathways, cytokine-mediated signaling pathways, and transcription factors.

What is the potential application value of CCL22 protein in treatment?

Studies have shown that by interfering with the interaction of the CCL22 protein and its receptor CCR4, tumor growth, improve inflammatory response, and regulate autoimmune diseases can be inhibited. These findings provide potential application value for the development of corresponding therapeutic strategies.

Are there inhibitors or antagonists that interact with the CCL22 protein?

A number of inhibitors or antagonists that interact with CCL22 proteins have been developed that block the binding of CCL22 to its receptor CCR4, thereby interfering with its biological function.

How to study the mechanism of action of CCL22 protein in specific diseases?

The mechanism of action of CCL22 protein in specific diseases can be studied through cell culture experiments, animal models, clinical samples, and related biochemical techniques.

How does the CCL22 protein relate to other chemokines?

There are complex interactions between CCL22 proteins and other chemokines. They may influence the migration and localization of immune cells by competing for receptors, mutual regulation of expression, etc.

What is the role of CCL22 protein in tumor immunotherapy?

Some studies have shown that in tumor immunotherapy, inhibiting the expression of CCL22 or interfering with its interaction with CCR4 can enhance the effect of immunotherapy and improve the survival rate of patients.