Active Recombinant Human CD155 protein, Fc-tagged

• Cat.No.: CD155-3057H
• Product Overview: Recombinant Human CD155 protein(Trp 21-Asn 343), fused with Fc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: Trp 21-Asn 343
• Form: Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human TIGIT, His Tag at 2 μg/mL (100 μL/well) can bind Human CD155, Fc Tag with a linear range of 8-128 ng/mL.Serial dilutions of Human TIGIT Neutralizing antibody were added into Human CD155, Fc Tag: Biotinylated Human TIGIT, Fc,Avitag binding reactions. The half maximal inhibitory concentration (IC50) is 0.06065 μg/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the C-terminus.The protein has a calculated MW of 61.7 kDa. The protein migrates as 95-105 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.