Active Recombinant Human CD19 Protein, Fc-tagged, Alexa Fluor 647 conjugated

• Cat.No.: CD19-3307HAF647
• Product Overview: Alexa Fluor 647 conjugated recombinant human CD19(Pro 20 - Pro 278) fused with with a human IgG1 with a human IgG1 Fc tag tag at C-terminal was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: Pro20-Pro278
• Form: Lyophilized
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Mouse anti-human CD19 Mab FMC63 or HIB19 10 μg/mL (100 μL/well) can bind Human CD19, Fc Tag with a linear ranger of 10-1000 ng/mL. Measured by its binding ability in a functional ELISA. Immobilized Human CD9, His Tag at 2 μg/mL (100 μL/well) can bind Human CD19, Fc Tag with a linear range of 0.05-0.5 μg/mL.
• Molecular Mass: Has a calculated MW of 55.1 kDa. DTT-reduced Protein migrates as 62-70 kDa in SDS-PAGE due to glycosylation.
• N-terminal Sequence Analysis: Pro 20
• Endotoxin: < 1.0 EU/ μg of the Human CD19 (20-278), Fc Tag by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 647 via amines
  Excitation = 650 nm
  Emission = 668 nm
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4 centigrade); After reconstitution under sterile conditions for 3 months (-70 centigrade).
• Storage Buffer: Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.
• Conjugation: Alexa Fluor 647

Gene Information

• Gene Name: CD19 CD19 molecule [ Homo sapiens ]
• Official Symbol: CD19
• Synonyms: CD19; CD19 molecule; CD19 antigen; B-lymphocyte antigen CD19; differentiation antigen CD19; T-cell surface antigen Leu-12; B-lymphocyte surface antigen B4; B4; CVID3; MGC12802
• Gene ID: 930
• mRNA Refseq: NM_001178098
• Protein Refseq: NP_001171569
• MIM: 107265
• UniProt ID: P15391