| Species : |
Human |
| Source : |
Insect Cells |
| Tag : |
Fc&His |
| Protein Length : |
19-152aa |
| Description : |
CD28, also known as T-cell-specific surface glycoprotein CD28 isoform 1, is one of the proteins expressed on T cells that provide co-stimulatory signals required for T cell activation and survival. T cell stimulation through CD28 in addition to the T-cell receptor (TCR) can provide a potent signal for the production of various interleukins (IL-6 in particular). CD28/B7 interaction has been shown to prevent apoptosis of activated T cells via the up-regulation of bcl-XL. It ligation has also been shown to regulate Th1/Th2 differentiation. Recombinant human CD28, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques. |
| Form : |
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol. |
| Bio-activity : |
Measured by its binding ability in a functional ELISA with Human CD80. The ED50 range ≤ 10ug/ml. |
| Molecular Mass : |
42.4 kDa |
| AA Sequence : |
NKILVKQ SPMLVAYDNA VNLSCKYSYN LFSREFRASL HKGLDSAVEV CVVYGNYSQQ LQVYSKTGFN CDGKLGNESV TFYLQNLYVN QTDIYFCKIE VMYPPPYLDN EKSNGTIIHV KGKHLCPSPL FPGPSKP |
| Endotoxin : |
< 1 EU per 1ug of protein (determined by LAL method). |
| Purity : |
> 90% |
| Applications : |
SDS-PAGE, Bioactivity |
| Storage : |
Can be stored at 2 centigrade to 8 centigrade for 1 week. For long term storage, aliquot and store at -20 centigrade to -80 centigrade. Avoid repeated freezing and thawing cycles. |
| Concentration : |
1mg/ml |
