Active Recombinant Human CD28 protein, hIgG-His-tagged

• Cat.No.: CD28-01H
• Product Overview: Recombinant Human CD28 protein, fused to hIgG-His-tag, was expressed in Insect cell.

Specification

• Description: CD28, also known as T-cell-specific surface glycoprotein CD28 isoform 1, is one of the proteins expressed on T cells that provide co-stimulatory signals required for T cell activation and survival. T cell stimulation through CD28 in addition to the T-cell receptor (TCR) can provide a potent signal for the production of various interleukins (IL-6 in particular). CD28/B7 interaction has been shown to prevent apoptosis of activated T cells via the up-regulation of bcl-XL. It ligation has also been shown to regulate Th1/Th2 differentiation. Recombinant human CD28, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
• Source: Insect cell
• Species: Human
• Tag: hIgG-His
• Form: Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol.
• Bio-activity: Measured by its binding ability in a functional ELISA with Human CD80. The ED50 range ≤ 10ug/ml.
• Molecular Mass: 42.4 kDa
• Protein length: 19-152aa
• AA Sequence: NKILVKQ SPMLVAYDNA VNLSCKYSYN LFSREFRASL HKGLDSAVEV CVVYGNYSQQ LQVYSKTGFN CDGKLGNESV TFYLQNLYVN QTDIYFCKIE VMYPPPYLDN EKSNGTIIHV KGKHLCPSPL FPGPSKP
• Endotoxin: < 1 EU per 1ug of protein (determined by LAL method).
• Purity: > 90%
• Applications: SDS-PAGE, Bioactivity
• Storage: Can be stored at 2 centigrade to 8 centigrade for 1 week. For long term storage, aliquot and store at -20 centigrade to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1mg/ml

Gene Information

• Gene Name: CD28 CD28 molecule [ Homo sapiens (human) ]
• Official Symbol: CD28
• Synonyms: T-cell-specific surface glycoprotein CD28 isoform 1; CD28; Tp44
• Gene ID: 940
• mRNA Refseq: NM_006139
• Protein Refseq: NP_006130
• MIM: 186760
• UniProt ID: P10747