Active Recombinant Human CD28 protein, hIgG-His-tagged

• Cat.No.: CD28-01H
• Product Overview: Recombinant Human CD28 protein, fused to hIgG-His-tag, was expressed in Insect cell.

Specification

• Description: CD28, also known as T-cell-specific surface glycoprotein CD28 isoform 1, is one of the proteins expressed on T cells that provide co-stimulatory signals required for T cell activation and survival. T cell stimulation through CD28 in addition to the T-cell receptor (TCR) can provide a potent signal for the production of various interleukins (IL-6 in particular). CD28/B7 interaction has been shown to prevent apoptosis of activated T cells via the up-regulation of bcl-XL. It ligation has also been shown to regulate Th1/Th2 differentiation. Recombinant human CD28, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
• Source: Insect cell
• Species: Human
• Tag: His&Fc
• Form: Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol.
• Bio-activity: Measured by its binding ability in a functional ELISA with Human CD80. The ED50 range ≤ 10ug/ml.
• Molecular Mass: 42.4 kDa
• Protein length: 19-152aa
• AA Sequence: NKILVKQ SPMLVAYDNA VNLSCKYSYN LFSREFRASL HKGLDSAVEV CVVYGNYSQQ LQVYSKTGFN CDGKLGNESV TFYLQNLYVN QTDIYFCKIE VMYPPPYLDN EKSNGTIIHV KGKHLCPSPL FPGPSKP
• Endotoxin: < 1 EU per 1ug of protein (determined by LAL method).
• Purity: > 90%
• Applications: SDS-PAGE, Bioactivity
• Storage: Can be stored at 2 centigrade to 8 centigrade for 1 week. For long term storage, aliquot and store at -20 centigrade to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1mg/ml

Gene Information

• Gene Name: CD28 CD28 molecule [ Homo sapiens (human) ]
• Official Symbol: CD28
• Synonyms: T-cell-specific surface glycoprotein CD28 isoform 1; CD28; Tp44
• Gene ID: 940
• mRNA Refseq: NM_006139
• Protein Refseq: NP_006130
• MIM: 186760
• UniProt ID: P10747

Reviews

08/24/2021

Its unique characteristics contribute to obtaining clear and detailed structural information, enabling researchers to unravel the intricate complexities of protein interactions and conformational changes.

08/24/2021

Researchers can confidently rely on its capabilities to generate precise and significant results, enhancing the understanding of protein function and dynamics.

08/06/2021

the CD28 protein's outstanding performance in ELISA and its utility in protein electron microscopy structure analysis make it a highly recommended tool for researchers.

Q&As

Can CD28 protein be used for personalized medicine?

CD28 protein expression and function may be relevant for personalized treatment strategies in autoimmune diseases and cancer.

What are the potential clinical applications of targeting CD28 protein?

Targeting CD28 protein may have therapeutic potential in treating autoimmune diseases, cancer immunotherapy, and organ transplantation.

How does CD28 protein impact regulatory T cells (Tregs)?

CD28 signaling is important for the function and maintenance of regulatory T cells, which play a role in immune tolerance and suppression of autoimmunity.

How does CD28 protein impact cancer immunotherapy?

CD28 protein is involved in enhancing the anti-tumor immune response, making it a potential target for cancer immunotherapy.

Are there any approved therapies targeting CD28 protein?

Currently, there are no FDA-approved therapies specifically targeting CD28 protein, but research is ongoing in this area.