Active Recombinant Human CD33 Protein, Fc-tagged, Atto 488 conjugated

• Cat.No.: CD33-27H
• Product Overview: Recombinant human Atto 488 conjugated Siglec-3/CD33 (Asp18-His259) (Val257Leu) protein with a human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in mouse myeloma cell line.

Specification

• Description: Enables protein phosphatase binding activity and sialic acid binding activity. Involved in several processes, including negative regulation of cytokine production; negative regulation of monocyte activation; and positive regulation of protein tyrosine phosphatase activity. Located in several cellular components, including Golgi apparatus; external side of plasma membrane; and peroxisome.
• Source: Mouse myeloma cell line
• Species: Human
• Tag: Fc
• Form: Disulfide-linked homodimer
  Labeled with Atto 488 via amines
  Excitation Wavelength: 501 nm
  Emission Wavelength: 523 nm
• Bio-activity: Measured by flow cytometry for its ability to bind anti-Human Siglec-3/CD33 Monoclonal Antibody conjugated fluorescent beads.
• Molecular Mass: 53.4 kDa
• N-terminal Sequence Analysis: Asp18
• Endotoxin: <1.0 EU/μg of the protein by the LAL method.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  6 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after opening.
  3 months, -20 to -70 centigrade under sterile conditions after opening.
• Storage Buffer: Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Shipping: The product is shipped with dry ice or equivalent.

Gene Information

• Gene Name: CD33 CD33 molecule [ Homo sapiens (human) ]
• Official Symbol: CD33
• Synonyms: CD33; CD33 molecule; p67; SIGLEC3; SIGLEC-3; myeloid cell surface antigen CD33; CD33 antigen (gp67); CD33 molecule transcript; gp67; sialic acid-binding Ig-like lectin 3
• Gene ID: 945
• mRNA Refseq: M23197
• Protein Refseq: AAA51948
• MIM: 159590
• UniProt ID: P20138

Reviews

11/14/2019

Quick dissolution, easy use.

08/27/2019

Excellent solubility, no aggregates.

02/27/2019

High concentration, very effective.

Q&As

What are the implications of CD33 polymorphisms in therapeutic targeting?

Certain polymorphisms in the CD33 gene can influence its expression on the cell surface, which might affect the efficacy of CD33-targeted therapies.

What is the primary biological function of CD33 in hematopoietic cells?

CD33 is a transmembrane receptor primarily expressed on cells of myeloid lineage and is involved in modulating cellular functions, including the regulation of cellular proliferation and differentiation.

How does CD33 expression vary among different leukocyte populations?

CD33 is predominantly expressed on myeloid cells, especially on immature myeloid cells. Its expression decreases as these cells differentiate and is typically low or absent on lymphoid cells.

How has CD33 been utilized as a therapeutic target in hematological malignancies?

CD33 has been targeted for therapeutic antibodies in AML. An example is gemtuzumab ozogamicin, which targets CD33 and delivers a cytotoxic agent specifically to leukemia cells expressing CD33.

How does CD33 signaling influence myeloid cell differentiation?

While the exact signaling pathways of CD33 are still under investigation, it is believed that CD33 signaling can influence the differentiation and function of myeloid cells, potentially playing a role in immune regulation.

What role does CD33 play in acute myeloid leukemia (AML)?

CD33 is commonly expressed on the surface of AML blasts, making it a potential target for therapeutic interventions in AML.

Are there known ligands that bind specifically to CD33?

The specific ligands for CD33 are not fully characterized, but it is believed to play a role in cell-cell interactions within the immune system.